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Molecular Human Reproduction Vol. 1, NUMBER 8 pp. 395-400, 1995
© European Society of Human Reproduction and Embryology 1995

research-article

Human oviductal cells produce high molecular weight factor(s) that improves the development of mouse embryo

L.P.S. Liu, S.T.H. Chan, P.C. Ho1 and W.S.B. Yeung1,2

Departments of Zoology University of Hong Kong, Hong Kong 1Obstetrics and Gynaecology University of Hong Kong, Hong Kong

To whom correspondence should be addressed at: 2To whom correspondence should be addressed at: Department of Obstetrics and Gynaecology, University of Hong Kong, Queen Mary Hospital, Hong Kong

The cocuhture effects of human oviductal cells on mouse embryo development in vitro were studied. Pronuclear stage mouse zygotes were cocultured with different cell types, or then cultured either in medium alone (control), spent medium from oviductal cell culture (conditioned medium) or high molecular weight fractions (>10 and>100 kDa) of the conditioned medium (reconstituted medium). Embryotrophic activities were compared between these groups in terms of percentage of morula and blastocyst formation, and cell count at the blastocyst stage. The mouse embryos developed better in oviductal cell cocutture than in fibroblast coculture and medium alone culture. Conditioned medium and its reconstituted medium also provided a significant enhancement of embryo development in vitro when compared with the control medium culture, suggesting the production of high molecular weight embryotrophic factor(s) by the oviductal cells. The high molecular weight embryotrophic activity accumulated with the duration of conditioning could be serially diluted, and was abolished by heat or trypsin treatment. Replacing bovine serum albumin with polyvinyl alcohol in the culture medium did not affect the production of this high molecular weight embryotrophic activity by oviductal cells.

coculture/embryos/embryotrophic factor/oviductal cells


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