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Molecular Human Reproduction Vol. 1, NUMBER 8 pp. 401-406, 1995
© European Society of Human Reproduction and Embryology 1995

research-article

Expression of transcription regulating genes in human preimplantation embryos

Burae Abdel-Rahman1, Morris Fiddler2, Daniel Rappolee1,2 and Eugene Pergament1,4

1Department of Obstetrics and Gynecology, Section of Reproductive Genetics, Northwestern University Medical School Prentice Women's Hospital and Maternity Center, 333 E. Superior Street, Suite 1566, Chicago, Illinois 2DePaul University School for New Learning, Chicago, Illinois 3Department of Cell and Molecular Biology Northwestern University Medical School, Chicago, Illinois, USA

To whom correspondence should be addressed at: 4To whom correspondence should be addressed

Utilizing a sensitive reverse transcriptase-polymerase chain reaction (RT-PCR) assay system, the time course of mRNA expression of two transcription regulators, OCT4 and OCT6, was assessed in individual preimplantation human embryos. Examination of ova with three pronuclei and 1-cell through blastocyst stage embryos revealed that OCT4 mRNA was continuously expressed between the time of fertilization and 10 + cell stages, whereas OCT6 mRNA expression was not observed until the 10+ cell stage. The difference in the time of expression of OCT4 mRNA and OCT6 mRNA indicates that the two genes play differential roles in human embryogenesis. Nucleotide sequence homology for OCT4 and OCT6 among mammalian organisms supports the concept that genetic elements determining developmental events during embryogenesis are conserved in evolution.

OCT4/OCT6/POU-domain/reverse transcriptase


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