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Mol. Hum. Reprod. Advance Access originally published online on October 15, 2004
Molecular Human Reproduction 2004 10(12):895-899; doi:10.1093/molehr/gah112
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Molecular Human Reproduction vol. 10 no. 12 © European Society of Human Reproduction and Embryology 2004; all rights reserved

Mechanical stretch of human uterine smooth muscle cells increases IL-8 mRNA expression and peptide synthesis

J.A.Z. Loudon1, S.R. Sooranna2, P.R. Bennett1 and M.R. Johnson2,3

Imperial College Parturition Research Group 1Institute of Reproductive and Developmental Biology, Hammersmith Hospital Campus DuCane Road, London W12 0NN and 2Department of Maternal Fetal Medicine, Chelsea and Westminster Hospital, 369 Fulham Road, London SW10 9NH, UK

3 To whom correspondence should be addressed. Email: mark.johnson{at}imperial.ac.uk

Labour is associated with increased synthesis of interleukin-8 (IL-8) by the fetal membranes and myometrium, which leads to an inflammatory infiltrate. Stretch has been shown to increase the expression of contraction-associated proteins in animal models of labour and in human myocytes in vitro. In this study, we tested the hypothesis that mechanical stretch of human myometrial cells increases IL-8 messenger ribonucleic acid (mRNA) expression. We isolated myocytes from non-pregnant women undergoing hysterectomy and pregnant women undergoing Caesarean section before and after the onset of labour. Myocytes in culture were subjected to stretch of varying intensity (6–16%) and duration (1 or 6 h) using the Flexercell system. IL-8 mRNA expression was lowest in myocytes from pregnant women not in labour, intermediate in those from non-pregnant women and greatest in those from pregnant women in labour. Stretch increased IL-8 mRNA expression independent of reproductive state. The stretch-induced increase in IL-8 mRNA expression was associated with higher IL-8 levels in the culture supernatant and enhanced promoter activity. These data suggest that stretch contributes to the increase in myometrial IL-8 synthesis associated with the onset of labour in humans.

Key words: interleukin-8/stretch/labour


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