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Mol. Hum. Reprod. Advance Access originally published online on October 22, 2004
Molecular Human Reproduction 2004 10(12):935-939; doi:10.1093/molehr/gah124
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Molecular Human Reproduction vol. 10 no. 12 © European Society of Human Reproduction and Embryology 2004; all rights reserved

Progesterone inhibits insulin-like growth factor binding protein-1 (IGFBP-1) production by explants of the Fallopian tube

S. Davies, M.C. Richardson1, F.W. Anthony, D. Mukhtar and I.T. Cameron

Maternal, Fetal and Neonatal Physiology, Developmental Origins of Health and Disease Division, University of Southampton, Level F (815), Princess Anne Hospital, Coxford Road, Southampton SO16 5YA, UK

1 To whom correspondence should be addressed. Email: mcr2{at}soton.ac.uk

The Fallopian tube provides the environment for early embryo growth, a process which is influenced by insulin-like growth factors (IGFs) in the tubal fluid. Whether the bioavailability of tubal IGFs is modulated by locally produced IGF-binding protein (IGFBP-1) is not clear. An explant culture system from human Fallopian tube mucosa was, therefore, developed enabling the potential for IGFBP-1 production by this tissue to be examined directly. Initial characterization of the system established that the explants maintained responsiveness to steroids. Thus, oviduct-specific glycoprotein production, a major product of the oviduct in vivo, continued to be made via an estrogen-sensitive pathway in the culture. The presence of mRNA for IGFBP-1 was established within the explants by the use of quantitative RT–PCR and IGFBP-1 protein was measured by enzyme-linked immunosorbent assay. Although insulin and estradiol had no consistent effect on IGFBP-1, addition of progesterone had a significant inhibitory effect on IGFBP-1 production, both at the mRNA and protein levels. A dose-range of progesterone revealed an incremental inhibitory effect of progesterone on IGFBP-1 output (maximal effect, 25–50 nmol/l), consistent with physiological inhibition of this process during the luteal phase. We suggest that progesterone might, therefore, play a role in controlling the bioavailability of IGFs to the embryo during early development within the Fallopian tube.

Key words: Fallopian tube/IGFBP-1/oviduct-specific glycoprotein/progesterone


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