Molecular Human Reproduction, Vol. 10, No. 2, pp. 91-97, 2004
© European Society of Human Reproduction and Embryology 2004
The role of 
5ß1-integrin in the IGF-I-induced migration of extravillous trophoblast cells during the process of implantation
Departments of 1Obstetrics and Gynecology and 2Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo, 181-8611, Japan and 3Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, 14115-111 Tehran, Iran
4 To whom correspondence should be addressed at: Department of Obstetrics and Gynecology, Kyorin University School of Medicine, Mitaka, Tokyo, 181-8611, Japan. e-mail: kabirs_m{at}yahoo.com
The role of integrins in the processes of adhesion and migration makes them attractive potential participants in the complex events of embryo implantation and placentation. Recently, the role of the 
vß3-integrin pathway was shown in the insulin-like growth factor-I (IGF-I)-stimulated migration of extravillous trophoblast (EVT) cells. This study was designed to investigate the role of 5ß1-integrin in this respect. Using cultured EVT cells, migration assays were carried out for IGF-I-treated or untreated cells in the presence or absence of the GRGDSP and GRGESP hexapeptides, IR3, and a blocking antibody against 5ß1-integrin. Immuno-electron microscopy and immunofluorescent staining were performed to localize the distribution of 5ß1- and vß3-integrins, Rab5a, paxillin, phospho-FAK (pFAK), and vinculin. The results showed that IGF-I-induced migration of EVT cells was abolished following treatment with GRGDSP hexapeptide, IR3, and a blocking antibody against 5ß1-integrin. Further, statistical analysis showed that the area-related numerical density of the 5ß1-integrin in the perinuclear regions was significantly higher than in the cell extensions. Immunocytochemical experiments demonstrated an up-regulation in internalization rate of 5ß1-integrin in IGF-I-stimulated EVT cells. Furthermore, 5ß1-integrin exhibited co-localization with Rab5a, but not with vß3-integrin, pFAK, paxillin, and vinculin at the focal adhesions of the EVT cells. Taken together, these findings suggest an essential role for 5ß1-integrin in IGF-I-promoted migration of EVT cells. It is possible therefore that IGF-I-induced internalization of 5ß1-integrin may be an important event during the migration of EVT cells in the complex processes of implantation and placentation.
Key words:
Key words: 5ß1-integrin/IGF-I/implantation/placentation/trophoblast migration
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Kabir-Salmani, M. N. Fukuda, M. Kanai-Azuma, N. Ahmed, S. Shiokawa, Y. Akimoto, K. Sakai, S. Nagamori, Y. Kanai, K. Sugihara, et al. The Membrane-Spanning Domain of CD98 Heavy Chain Promotes {alpha}v{beta}3 Integrin Signals in Human Extravillous Trophoblasts Mol. Endocrinol., March 1, 2008; 22(3): 707 - 715. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-K. Shields, C. Nicola, and C. Chakraborty Rho Guanosine 5'-Triphosphatases Differentially Regulate Insulin-Like Growth Factor I (IGF-I) Receptor-Dependent and -Independent Actions of IGF-II on Human Trophoblast Migration Endocrinology, October 1, 2007; 148(10): 4906 - 4917. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Tartakover-Matalon, N. Cherepnin, M. Kuchuk, L. Drucker, I. Kenis, A. Fishman, M. Pomeranz, and M. Lishner Impaired migration of trophoblast cells caused by simvastatin is associated with decreased membrane IGF-I receptor, MMP2 activity and HSP27 expression Hum. Reprod., April 1, 2007; 22(4): 1161 - 1167. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. N. Sferruzzi-Perri, J. A. Owens, P. Standen, R. L. Taylor, G. K. Heinemann, J. S. Robinson, and C. T. Roberts Early treatment of the pregnant guinea pig with IGFs promotes placental transport and nutrient partitioning near term Am J Physiol Endocrinol Metab, March 1, 2007; 292(3): E668 - E676. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Shukla, X. Coumoul, L. Cao, R.-H. Wang, C. Xiao, X. Xu, S. Ando, S. Yakar, D. LeRoith, and C. Deng Absence of the full-length breast cancer-associated gene-1 leads to increased expression of insulin-like growth factor signaling axis members. Cancer Res., July 15, 2006; 66(14): 7151 - 7157. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kabir-Salmani, S. Shiokawa, Y. Akimoto, K. Sakai, K. Sakai, and M. Iwashita Tissue Transglutaminase at Embryo-Maternal Interface J. Clin. Endocrinol. Metab., August 1, 2005; 90(8): 4694 - 4702. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Lynch, P. I. Vodyanik, D. Boettiger, and M. A. Guvakova Insulin-like Growth Factor I Controls Adhesion Strength Mediated by {alpha}5{beta}1 Integrins in Motile Carcinoma Cells Mol. Biol. Cell, January 1, 2005; 16(1): 51 - 63. [Abstract] [Full Text] [PDF]
|
|