Skip Navigation


Mol. Hum. Reprod. Advance Access originally published online on January 29, 2004
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
10/4/283    most recent
gah038v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (22)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Hu, D. G.
Right arrow Articles by Hussey, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hu, D. G.
Right arrow Articles by Hussey, N.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Molecular Human Reproduction, Vol. 10, No. 4, pp. 283-289, 2004
© European Society of Human Reproduction and Embryology 2004

Aneuploidy detection in single cells using DNA array-based comparative genomic hybridization

Dong Gui Hu, Graham Webb and Nicole Hussey1

Reproductive Medicine Unit, Department of Obstetrics and Gynaecology, University of Adelaide, The Queen Elizabeth Hospital, Woodville Rd, Woodville, South Australia, 5011, Australia

1 To whom correspondence should be addressed. e-mail: nicole.hussey{at}adelaide.edu.au

The use of metaphase comparative genomic hybridization (CGH) to screen all human chromosomes for aneuploidy in preimplantation embryos is hindered by the time required to perform the analysis. We report in this paper a novel approach to manufacture a DNA microarray for CGH for the detection of aneuploidy in single cells. We spotted human chromosome-specific libraries on glass slides that were depleted of repetitive sequences and tested our array CGH method in 14 experiments using either single male and/or single female lymphocytes. For the autosomes, the mean normalized ratios were all close to the expected ratio of 1.0 with overall 300/308 (97%) of the normalized ratios falling within the range 0.75 to 1.25. It was possible to deduce the correct copy number of the X chromosome in 13/14 (92.9%) separate array CGH experiments but the Y chromosome in only 4/14 (29%). We tested our microarray CGH method on a single fibroblast from each of three cell lines containing a specific chromosome aneuploidy (trisomy 13, 15 or 18) and in each case our microarray analysis was able to obtain a diagnosis based on the fact that the aneuploid chromosome gave the highest ratio (1.32, 1.27 and 1.27 respectively) with the ratios of all other chromosomes falling within the range 0.75–1.25. Requiring just 30 h, our method may be more suitable for PGD aneuploidy screening than metaphase CGH.

Key words: Key words: aneuploidy screening/array CGH/microarray/preimplantation genetic diagnosis/single cell PCR


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
J. B. Geigl, A. C. Obenauf, J. Waldispuehl-Geigl, E. M. Hoffmann, M. Auer, M. Hormann, M. Fischer, Z. Trajanoski, M. A. Schenk, L. O. Baumbusch, et al.
Identification of small gains and losses in single cells after whole genome amplification on tiling oligo arrays
Nucleic Acids Res., June 18, 2009; (2009) gkp526v1.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
D. Wells, S. Alfarawati, and E. Fragouli
Use of comprehensive chromosomal screening for embryo assessment: microarrays and CGH
Mol. Hum. Reprod., December 1, 2008; 14(12): 703 - 710.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
A. Obradors, E. Fernandez, M. Oliver-Bonet, M. Rius, A. de la Fuente, D. Wells, J. Benet, and J. Navarro
Birth of a healthy boy after a double factor PGD in a couple carrying a genetic disease and at risk for aneuploidy: Case Report
Hum. Reprod., August 1, 2008; 23(8): 1949 - 1956.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. Fuhrmann, O. Schmidt-Kittler, N. H. Stoecklein, K. Petat-Dutter, C. Vay, K. Bockler, R. Reinhardt, T. Ragg, and C. A. Klein
High-resolution array comparative genomic hybridization of single micrometastatic tumor cells
Nucleic Acids Res., April 1, 2008; 36(7): e39 - e39.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H. Fiegler, J. B. Geigl, S. Langer, D. Rigler, K. Porter, K. Unger, N. P. Carter, and M. R. Speicher
High resolution array-CGH analysis of single cells
Nucleic Acids Res., February 16, 2007; 35(3): e15 - e15.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
P. Donoso, C. Staessen, B.C.J.M. Fauser, and P. Devroey
Current value of preimplantation genetic aneuploidy screening in IVF
Hum. Reprod. Update, January 1, 2007; 13(1): 15 - 25.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. Le Caignec, C. Spits, K. Sermon, M. De Rycke, B. Thienpont, S. Debrock, C. Staessen, Y. Moreau, J.-P. Fryns, A. Van Steirteghem, et al.
Single-cell chromosomal imbalances detection by array CGH.
Nucleic Acids Res., January 1, 2006; 34(9): e68 - e68.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Knijnenburg, M. van der Burg, P. Nilsson, H. K. P. van Amstel, H. Tanke, and K. Szuhai
Rapid detection of genomic imbalances using micro-arrays consisting of pooled BACs covering all human chromosome arms
Nucleic Acids Res., October 12, 2005; 33(18): e159 - e159.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
L. Wilton
Preimplantation genetic diagnosis and chromosome analysis of blastomeres using comparative genomic hybridization
Hum. Reprod. Update, January 1, 2005; 11(1): 33 - 41.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.