Skip Navigation


Mol. Hum. Reprod. Advance Access originally published online on April 20, 2004
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
10/6/467    most recent
gah061v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (13)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Paulasova, P.
Right arrow Articles by Pellestor, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Paulasova, P.
Right arrow Articles by Pellestor, F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Molecular Human Reproduction, Vol. 10, No. 6, pp. 467-472, 2004
© European Society of Human Reproduction and Embryology 2004

The peptide nucleic acids as probes for chromosomal analysis: application to human oocytes, polar bodies and preimplantation embryos

P. Paulasova1, B. Andréo2, J. Diblik1, M. Macek1 and F. Pellestor2,3

1Laboratory of Assisted Reproduction, Motol Hospital, Vuvalu 84, 150 06 Praha 5, Czech Republic and 2CNRS UPR 1142, Institut de Génétique Humaine, 141 rue de la Cardonille, F-34396 Montpellier Cedex 5, France

3 To whom correspondence should be addressed. e-mail: franck.pellestor{at}igh.cnrs.fr

Peptide nucleic acids (PNA) are synthetic DNA mimics based on an uncharged polyamide backbone, which hybridize with complementary DNA with high affinity and specificity. PNA have recently become recognized as efficient tools for in situ chromosomal identification. In the present study, this new approach has been tried on isolated human oocytes, polar bodies and blastomeres. Using centromeric PNA probes specific for chromosomes 1, 4, 9, 16, X and Y, we tested multicolour labelling PNA reaction on 27 oocytes and 23 blastomeres. Sequential PNA hybridization was performed on five oocytes and combined PNA and fluorescence in situ hybridization (FISH) reactions on two oocytes. Both the rates and the types of abnormalities observed are in agreement with results from previous FISH studies. This preliminary study indicates that PNA probes allow a reliable chromosomal analysis in isolated human oocytes and blastomeres and consequently might provide an interesting adjunct to FISH for diagnostic analysis.

Key words: aneuploidy/blastomere/oocyte/peptide nucleic acids/polar body


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum ReprodHome page
I.E. Agerholm, S. Ziebe, B. Williams, C. Berg, D.G. Cruger, G. B. Petersen, and S. Kolvraa
Sequential FISH analysis using competitive displacement of labelled peptide nucleic acid probes for eight chromosomes in human blastomeres
Hum. Reprod., April 1, 2005; 20(4): 1072 - 1077.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
F. Pellestor, P. Paulasova, M. Macek, and S. Hamamah
The Use of Peptide Nucleic Acids for In Situ Identification of Human Chromosomes
J. Histochem. Cytochem., March 1, 2005; 53(3): 395 - 400.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
F. Pellestor, T. Anahory, and S. Hamamah
The chromosomal analysis of human oocytes. An overview of established procedures
Hum. Reprod. Update, January 1, 2005; 11(1): 15 - 32.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.