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Mol. Hum. Reprod. Advance Access originally published online on July 8, 2004
Molecular Human Reproduction 2004 10(9):623-628; doi:10.1093/molehr/gah083
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Molecular Human Reproduction vol. 10 no. 9 © European Society of Human Reproduction and Embryology 2004; all rights reserved

Basic fibroblast growth factor expression in isolated small human ovarian follicles

J.H. Quennell, J-A.L. Stanton and P.R. Hurst1

Department of Anatomy and Structural Biology, School of Medical Sciences, University of Otago, P.O.Box 56, Dunedin, 9001, New Zealand

1 To whom correspondence should be addressed. Email: peter.hurst{at}stonebow.otago.ac.nz

Basic fibroblast growth factor (bFGF) is involved in cell proliferation, differentiation, and angiogenesis. It has long been known that bFGF acts as a powerful mitogen for various mammalian granulosa cells in culture. To investigate the possible involvement of bFGF expression in follicle initiation and growth in vivo, we performed nested RT–PCR on ovarian cortical biopsies and quantitative PCR on human follicle populations isolated by laser capture microdissection. Using morphological criteria, follicles were characterized as putative non-growing, primary, or small secondary. RNA was extracted from samples, reverse-transcribed, and relative gene expression levels determined with TaqManTM real-time PCR, using 18S rRNA as the endogenous control. Results confirmed bFGF expression in human adult ovarian cortex, and in the isolated follicles a down-regulation of bFGF mRNA was evident as small follicles develop. This study demonstrates a possible relationship between bFGF mRNA expression and follicle development.

Key words: bFGF/follicle/laser microdissection/ovary/real-time


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