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Mol. Hum. Reprod. Advance Access originally published online on December 19, 2005
Molecular Human Reproduction 2005 11(11):825-831; doi:10.1093/molehr/gah239
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© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Ikaros is expressed in human extravillous trophoblasts and involved in their migration and invasion

E. Yamamoto1, T. Ito1,5, A. Abe2, F. Sido1, K. Ino1, A. Itakura1, S. Mizutani3, S. Dovat4, S. Nomura1 and F. Kikkawa1

1Department of Obstetrics and Gynecology, 2Department of Hematology, 3Department of Clinical applied research of Proteases, Nagoya University Graduate School of Medicine, Nagoya, Japan and 4Pediatrics Hematology/Oncology, University of Wisconsin Medical School, Madison, WI, USA

5 To whom correspondence should be addressed at: Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan. E-mail: itoto{at}med.nagoya-u.ac.jp

The transcriptional factor Ikaros was originally found to function as a key regulator of lymphocyte differentiation. In addition, we have reported that Ikaros regulates the human placental leucine aminopeptidase (P-LAP)/insulin-regulated aminopeptidase (IRAP) gene in choriocarcinoma trophoblastic cells, suggesting that Ikaros might be involved in placental development, while even its presence in human placenta remains undetermined. We therefore sought to clarify the location and roles of Ikaros in human placenta. Immunohistochemical analysis showed modest Ikaris expression in syncytium, and intense expression in extravillous trophoblasts (EVTs) in first trimester placenta. Western blot analysis showed that villous trophoblasts principally expressed Ikaros-2/3, while Ikaros-x (Ikx) was predominantly expressed in cultured EVTs. Furthermore, to investigate the functional role of Ikx in EVTs, the EVT cell line HTR-8/SVneo was infected with a retrovirus vector expressing the hemagglutinin (HA)-tagged dominant negative isoform Ikaros-6 (Ik6), which prevents the DNA-binding activity of Ikx. Antibody against HA showed successful transduction of Ik6 in HTR-8/SVneo cells on immunocytochemistry and Western blotting. Transduction of Ik6 significantly reduced the migratory and invasive abilities of HTR-8/SVneo cells. These results suggest that Ikx is involved in migration and invasion of EVTs in early placentation.

Key words: extravillous trophoblasts/human/Ikaros/placentation/transcriptional factor


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