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Mol. Hum. Reprod. Advance Access originally published online on December 22, 2004
Molecular Human Reproduction 2005 11(2):87-92; doi:10.1093/molehr/gah141
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Molecular Human Reproduction vol. 11 no. 2 © European Society of Human Reproduction and Embryology 2004; all rights reserved

Psoriasin, a calcium-binding protein with chemotactic properties is present in the third trimester amniotic fluid

S. Porre1, S. Heinonen2, R. Mäntyjärvi1, M. Rytkönen-Nissinen1, O. Perola1, J. Rautiainen1 and T. Virtanen1,3

1Department of Clinical Microbiology, University of Kuopio, FIN-70211, and 2Department of Obstetrics and Gynaecology, Kuopio University Hospital, FIN-70210, Kuopio, Finland

3 To whom correspondence should be addressed at: Department of Clinical Microbiology, University of Kuopio, P.O.B. 1627, FIN-70211 Kuopio, Finland. Email: tuomas.virtanen{at}uku.fi

Psoriasin is a small calcium-binding protein first found in psoriatic lesions and also up-regulated in other inflammatory skin diseases and cancer tissues. Psoriasin is also present in the fetal epithelial cells. Its biological function is unclear, but there is both in vitro and in vivo evidence for its chemokine-like activity. The aim of the present study was to find whether psoriasin could be found in the amniotic fluid and thus could have long-range immunobiological effects. Two recombinant psoriasins were prepared, one in Escherichia coli, the other one in Pichia pastoris. The former was used to produce a rabbit antiserum against psoriasin. Fractionation of full-term amniotic fluids with polyacrylamide gel electrophoresis (PAGE) and gel filtration associated with immunodetection with the antiserum were used to identify a protein compatible with the size of psoriasin. The identity of psoriasin was further verified by mass spectrometric analysis. Expression of psoriasin in cells of the amniotic membranes was detected with nested RT–PCR. Because of its chemokine-like activity, psoriasin present in the amniotic fluid might have consequential immunobiological effects during the fetal development.

Key words: amniotic fluid/calcium-binding protein/psoriasin/recombinant protein


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