Skip Navigation


Mol. Hum. Reprod. Advance Access originally published online on February 4, 2005
Molecular Human Reproduction 2005 11(3):195-205; doi:10.1093/molehr/gah150
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
11/3/195    most recent
gah150v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (29)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Horcajadas, J. A.
Right arrow Articles by Simón, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Horcajadas, J. A.
Right arrow Articles by Simón, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Molecular Human Reproduction Vol. 11 No. 3 © European Society of Human Reproduction and Embryology 2005; all rights reserved

Effect of controlled ovarian hyperstimulation in IVF on endometrial gene expression profiles

José Antonio Horcajadas1, Anne Riesewijk2, Jan Polman2, Roselinde van Os2, Antonio Pellicer1, Sietse Mosselman2 and Carlos Simón1,3

1Instituto Valenciano de Infertilidad Foundation (FIVI)-Valencia University, Spain, 2NV Organon, Department of Pharmacology, Oss, The Netherlands

3 To whom correspondence should be addressed at: C/Guadassuar, 1, 46015 Valencia, Spain. Email: csimon{at}ivi.es José Antonio Horcajadas and Anne Riesewijk contributed equally to this study

Controlled ovarian hyperstimulation (COH) used in IVF produces lower implantation rates per embryo transferred compared to natural cycles utilized in ovum donation, suggesting a suboptimal endometrial development. Endometrial receptivity has recently been investigated in natural menstrual cycles with the aid of microarray technology. The aim of this study is to investigate the impact of COH using urinary gonadotrophins with a long protocol with GnRH agonists without progesterone supplementation (similar to the natural cycle) on endometrial gene expression profiles during the window of implantation by comparing the profiles at day hCG+7 of COH versus LH+7 of a previous natural cycle in the same women. For this purpose we have used microarray technology by Affymetrix (GeneChip HG_U133A), which allows more than 22 000 genes to be tested simultaneously. Results were validated by semi-quantitative PCR and quantitative PCR experiments. We found that more than 200 genes showed a differential expression of more than 3-fold when COH and normal cycles were compared at hCG+7 versus LH+7. We simultaneously re-analysed the LH+2 versus LH+7 endometrial gene expression profiles in previous natural cycles in the same subject using this specific GeneChip, the results obtained were consistent with our own published results. This is the first time that gene expression profiles of the endometrium during COH are reported. The large degree of gene expression disturbance is surprising and highlights the need for further efforts to optimize COH protocols.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum ReprodHome page
Y. Liu, S. P. Kodithuwakku, P.-Y. Ng, J. Chai, E. H.Y. Ng, W. S.B. Yeung, P.-C. Ho, and K.-F. Lee
Excessive ovarian stimulation up-regulates the Wnt-signaling molecule DKK1 in human endometrium and may affect implantation: an in vitro co-culture study
Hum. Reprod., February 1, 2010; 25(2): 479 - 490.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
A. Allegra, A. Marino, F. Coffaro, A. Lama, G. Rizza, P. Scaglione, F. Sammartano, A. Santoro, and A. Volpes
Is there a uniform basal endometrial gene expression profile during the implantation window in women who became pregnant in a subsequent ICSI cycle?
Hum. Reprod., October 1, 2009; 24(10): 2549 - 2557.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
D. Haouzi, S. Assou, K. Mahmoud, S. Tondeur, T. Reme, B. Hedon, J. De Vos, and S. Hamamah
Gene expression profile of human endometrial receptivity: comparison between natural and stimulated cycles for the same patients
Hum. Reprod., June 1, 2009; 24(6): 1436 - 1445.
[Abstract] [Full Text] [PDF]

Home page
 Eur J EndocrinolHome page
M Seppala, H Koistinen, R Koistinen, L Hautala, P C Chiu, and W S Yeung
Glycodelin in reproductive endocrinology and hormone-related cancer
Eur. J. Endocrinol., February 1, 2009; 160(2): 121 - 133.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
J. A. Horcajadas, P. Minguez, J. Dopazo, F. J. Esteban, F. Dominguez, L. C. Giudice, A. Pellicer, and C. Simon
Controlled Ovarian Stimulation Induces a Functional Genomic Delay of the Endometrium with Potential Clinical Implications
J. Clin. Endocrinol. Metab., November 1, 2008; 93(11): 4500 - 4510.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
J. A. Horcajadas, E. Goyri, M. A. Higon, J. A. Martinez-Conejero, P. Gambadauro, G. Garcia, M. Meseguer, C. Simon, and A. Pellicer
Endometrial Receptivity and Implantation Are Not Affected by the Presence of Uterine Intramural Leiomyomas: A Clinical and Functional Genomics Analysis
J. Clin. Endocrinol. Metab., September 1, 2008; 93(9): 3490 - 3498.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
N. A. Bersinger, D. M. Wunder, M. H. Birkhauser, and M. D. Mueller
Gene expression in cultured endometrium from women with different outcomes following IVF
Mol. Hum. Reprod., August 1, 2008; 14(8): 475 - 484.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
H.-F. Xia, J. Sun, Q.-H. Sun, Y. Yang, and J.-P. Peng
Implantation-associated gene-1 (Iag-1): a novel gene involved in the early process of embryonic implantation in rat
Hum. Reprod., July 1, 2008; 23(7): 1581 - 1593.
[Abstract] [Full Text] [PDF]

Home page
 Reproductive SciencesHome page
N. S. Macklon, M. H. van der Gaast, A. Hamilton, B. C. J. M. Fauser, and L. C. Giudice
The Impact of Ovarian Stimulation With Recombinant FSH in Combination With GnRH Antagonist on the Endometrial Transcriptome in the Window of Implantation
Reproductive Sciences, April 1, 2008; 15(4): 357 - 365.
[Abstract] [PDF]

Home page
 Hum Reprod UpdateHome page
K. Diedrich, B.C.J.M. Fauser, P. Devroey, G. Griesinger, and on behalf of the Evian Annual Reproduction (EVAR)
The role of the endometrium and embryo in human implantation
Hum. Reprod. Update, July 1, 2007; 13(4): 365 - 377.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. R. A. Sherwin, A. M. Sharkey, P. Cameo, P. M. Mavrogianis, R. D. Catalano, S. Edassery, and A. T. Fazleabas
Identification of Novel Genes Regulated by Chorionic Gonadotropin in Baboon Endometrium during the Window of Implantation
Endocrinology, February 1, 2007; 148(2): 618 - 626.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
J.A. Horcajadas, A. Pellicer, and C. Simon
Wide genomic analysis of human endometrial receptivity: new times, new opportunities
Hum. Reprod. Update, January 1, 2007; 13(1): 77 - 86.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
R.M. Sibug, N. Datson, A.M.I. Tijssen, M. Morsink, J. de Koning, E.R. de Kloet, and F.M. Helmerhorst
Effects of urinary and recombinant gonadotrophins on gene expression profiles during the murine peri-implantation period
Hum. Reprod., January 1, 2007; 22(1): 75 - 82.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
H.-C. Ruan, X.-M. Zhu, Q. Luo, A.-X. Liu, Y.-L. Qian, C.-Y. Zhou, F. Jin, H.-F. Huang, and J.-Z. Sheng
Ovarian stimulation with GnRH agonist, but not GnRH antagonist, partially restores the expression of endometrial integrin beta3 and leukaemia-inhibitory factor and improves uterine receptivity in mice
Hum. Reprod., October 1, 2006; 21(10): 2521 - 2529.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
T. Strowitzki, A. Germeyer, R. Popovici, and M. von Wolff
The human endometrium as a fertility-determining factor
Hum. Reprod. Update, September 1, 2006; 12(5): 617 - 630.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
L. B. Romundstad, P. R. Romundstad, A. Sunde, V. von During, R. Skjaerven, and L. J. Vatten
Increased risk of placenta previa in pregnancies following IVF/ICSI; a comparison of ART and non-ART pregnancies in the same mother
Hum. Reprod., September 1, 2006; 21(9): 2353 - 2358.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
J. A. Horcajadas, A. M. Sharkey, R. D. Catalano, J. R. A. Sherwin, F. Dominguez, L. A. Burgos, A. Castro, M. R. Peraza, A. Pellicer, and C. Simon
Effect of an Intrauterine Device on the Gene Expression Profile of the Endometrium
J. Clin. Endocrinol. Metab., August 1, 2006; 91(8): 3199 - 3207.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
N. S. Macklon, R. L. Stouffer, L. C. Giudice, and B. C. J. M. Fauser
The Science behind 25 Years of Ovarian Stimulation for in Vitro Fertilization
Endocr. Rev., April 1, 2006; 27(2): 170 - 207.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Talbi, A. E. Hamilton, K. C. Vo, S. Tulac, M. T. Overgaard, C. Dosiou, N. Le Shay, C. N. Nezhat, R. Kempson, B. A. Lessey, et al.
Molecular Phenotyping of Human Endometrium Distinguishes Menstrual Cycle Phases and Underlying Biological Processes in Normo-Ovulatory Women
Endocrinology, March 1, 2006; 147(3): 1097 - 1121.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
C. Simon, J. Oberye, J. Bellver, C. Vidal, E. Bosch, J.A. Horcajadas, C. Murphy, S. Adams, A. Riesewijk, B. Mannaerts, et al.
Similar endometrial development in oocyte donors treated with either high- or standard-dose GnRH antagonist compared to treatment with a GnRH agonist or in natural cycles
Hum. Reprod., December 1, 2005; 20(12): 3318 - 3327.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
C. A White and L. A Salamonsen
A guide to issues in microarray analysis: application to endometrial biology
Reproduction, July 1, 2005; 130(1): 1 - 13.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.