Skip Navigation

Molecular Human Reproduction 2005 11(4):307-317; doi:10.1093/molehr/gah163
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (10)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Horowitz, E.
Right arrow Articles by Strauss, J. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Horowitz, E.
Right arrow Articles by Strauss, J. F., III
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Molecular Human Reproduction Vol.11 No.4 © European Society of Human Reproduction and Embryology 2005; all rights reserved

Patterns of expression of sperm flagellar genes: early expression of genes encoding axonemal proteins during the spermatogenic cycle and shared features of promoters of genes encoding central apparatus proteins*

Eran Horowitz1{dagger}, Zhibing Zhang1{dagger}, Brian H. Jones1, Stuart B. Moss1, Clement Ho1, Jennifer R. Wood1, Xingmei Wang2, Mary D. Sammel2 and Jerome F. Strauss, III1,3

1Center for Research on Reproduction and Women's Health and 2Center for Clinical Epidemiology and Biostatistics, University of Pennsylvania Medical Center, Philadelphia, PA 19104, USA

3 To whom correspondence should be addressed at: Center for Research on Reproduction and Women's Health, 1354 BRB II/III, 421 Curie Blvd, Philadelphia, PA 19104, USA. E-mail: jfs3{at}mail.med.upenn.edu

Sperm are motile cells. Thus, a significant component of the spermatogenic cycle is devoted to the formation of flagellum, a process that must be coordinated to insure proper construction. To document the temporal pattern of flagellar gene expression, we employed real-time PCR to assess changes in accumulation of a cohort of genes encoding axoneme, outer dense fibre (ODF) and fibrous sheath (FS) proteins during the first wave of spermatogenesis in the mouse. Axoneme genes were expressed first at the pachytene spermatocyte stage, followed by expression of transcripts encoding ODF and FS components. However, there were differences among these families with respect to the time of initial expression and the rate of mRNA accumulation. To gain understanding of factors that determine these patterns of expression, we cloned the promoters of three axoneme central apparatus genes (Pf6, Spag6 and Pf20). These promoters shared common features including the absence of a TATA box, and putative binding sites for several factors implicated in spermatogenesis (CREB/CREM, SOX17 and SPZ1) as well as ciliogenesis (FOXJ1). Collectively, our findings demonstrate a sequential pattern of expression of flagellar component genes, differential times of expression or rates of transcript accumulation within each class and shared promoter features within a class.

Key words: Mouse/PF6/PF20/SPAG6

* DNA sequences described in this report have been deposited in GenBank (accession nos. AY742711, AY742709, AY792595). {dagger}Authors Eran Horowitz and Zhibing Zhang contributed equally to this work.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
Z. Zhang, X. Shen, D. R. Gude, B. M. Wilkinson, M. J. Justice, C. J. Flickinger, J. C. Herr, E. M. Eddy, and J. F. Strauss III
MEIG1 is essential for spermiogenesis in mice
PNAS, October 6, 2009; 106(40): 17055 - 17060.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
Z. Zhang, X. Shen, B. H Jones, B. Xu, J. C Herr, and J. F Strauss III
Phosphorylation of Mouse Sperm Axoneme Central Apparatus Protein SPAG16L by a Testis-Specific Kinase, TSSK2
Biol Reprod, July 1, 2008; 79(1): 75 - 83.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
S. Assou, T. Anahory, V. Pantesco, T. Le Carrour, F. Pellestor, B. Klein, L. Reyftmann, H. Dechaud, J. De Vos, and S. Hamamah
The human cumulus-oocyte complex gene-expression profile
Hum. Reprod., July 1, 2006; 21(7): 1705 - 1719.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
Z. Zhang, I. Kostetskii, W. Tang, L. Haig-Ladewig, R. Sapiro, Z. Wei, A. M. Patel, J. Bennett, G. L. Gerton, S. B. Moss, et al.
Deficiency of SPAG16L Causes Male Infertility Associated with Impaired Sperm Motility
Biol Reprod, April 1, 2006; 74(4): 751 - 759.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Sironen, B. Thomsen, M. Andersson, V. Ahola, and J. Vilkki
An intronic insertion in KPL2 results in aberrant splicing and causes the immotile short-tail sperm defect in the pig
PNAS, March 28, 2006; 103(13): 5006 - 5011.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
A. M. Bieber, L. Marcon, B. F. Hales, and B. Robaire
Effects of Chemotherapeutic Agents for Testicular Cancer on the Male Rat Reproductive System, Spermatozoa, and Fertility
J Androl, March 1, 2006; 27(2): 189 - 200.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.