Mol. Hum. Reprod. Advance Access originally published online on March 11, 2005
Molecular Human Reproduction 2005 11(5):345-349; doi:10.1093/molehr/gah162
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The macrophage stimulating protein/RON system: a potential novel target for prevention and treatment of endometriosis
1Department of Gynecology, Polyclinique de l'Hôtel-Dieu, CHU, Clermont-Ferrand, France, 2Department of Obstetrics and Gynecology, Tohoku University Graduate School of Medicine, Sendai, Japan and 3Department of Pathology, Hôtel-Dieu, CHU, Clermont-Ferrand, France
4 To whom correspondence should be addressed at: Department of Gynecology, Polyclinique de l'Hôtel-Dieu, CHU Clermont-Ferrand, Bd. Léon Malfreyt, 63058, Clermont-Ferrand, Cedex 1, France. Email: sachikoma{at}aol.com
Our recent DNA microarray analysis using tissue obtained by laser capture microdissection (LCM) identified up-regulation of RON (a tyrosine kinase receptor) during the late secretory phase in eutopic endometrial epithelial cells from patients with deep endometriosis compared with control endometrium from women with macroscopically normal pelvic cavities. In the present study, we further investigated mRNA expression of RON and its ligand, macrophage stimulating protein (MSP), in deep endometriotic lesions, eutopic endometrium from patients with deep endometriosis and control endometrium by using LCM and quantitative real-time RT–PCR. MSP mRNA expression in endometrial epithelial cells was significantly up-regulated in endometriosis patients during the late secretory phase compared with expression in controls. Furthermore, we detected up-regulation of MSP mRNA in ectopic endometrial epithelial cells compared with matched eutopic endometrial epithelial cells within the same patients regardless of the menstrual phase. MSP has an intrinsically dual functional nature through its receptor RON—it is a trophic cytokine preventing apoptosis and a scatter factor promoting invasion, both of which may be necessary for the initial development and growth of endometriosis. The present findings suggest that the MSP/RON system may be involved in the pathophysiology of endometriosis.
Key words: endometriosis/endometrium/laser capture microdissection/macrophage stimulating protein/RON
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