Skip Navigation


Mol. Hum. Reprod. Advance Access originally published online on April 1, 2005
Molecular Human Reproduction 2005 11(5):365-372; doi:10.1093/molehr/gah165
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
11/5/365    most recent
gah165v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (1)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Chakravarty, S.
Right arrow Articles by Gupta, S. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chakravarty, S.
Right arrow Articles by Gupta, S. K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Molecular Human Reproduction © The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions{at}oupjournals.org

Baculovirus-expressed recombinant human zona pellucida glycoprotein-B induces acrosomal exocytosis in capacitated spermatozoa in addition to zona pellucida glycoprotein-C

Sanchita Chakravarty1, K. Suraj1 and Satish Kumar Gupta1,2

1Gamete Antigen Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi-110 067, India

2 To whom correspondence should be addressed at: Gamete Antigen Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi-110 067, India. Email: skgupta{at}nii.res.in

To facilitate our understanding of the role of zona pellucida glycoproteins during fertilization in humans, recombinant human zona pellucida glycoprotein-A (hZPA), -B (hZPB) and -C (hZPC) were obtained by using Escherichia coli and baculovirus expression systems. Analysis by SDS-PAGE and Western blot of the Ni-NTA affinity purified recombinant proteins revealed that the baculovirus-expressed hZPA, hZPB and hZPC have an apparent molecular weight of ~110, ~70–75 and ~65 kDa, respectively, as compared to ~80, ~65 and ~50 kDa of the respective E. coli-expressed proteins. Lectin binding studies revealed that the baculovirus-expressed recombinant zona proteins were glycosylated. Major oligosaccharides were represented by strong reactivity with Concanavalin A (mannose {alpha} 1–3 or mannose {alpha} 1–6 residues) and Jacalin ({alpha}-O glycosides of Gal or GalNAc moieties). A significant increase in acrosomal exocytosis was observed when capacitated human sperm were incubated in vitro with baculovirus-expressed hZPB (P=0.0005) and hZPC (P=0.0005) The E. coli-expressed hZPB, hZPC and baculovirus-expressed hZPA failed to induce any significant increase (P>0.05) in acrosome reaction. In contrast to hZPC, the acrosome reaction induced by recombinant hZPB was not inhibited by pertussis toxin. These studies, for the first time, have demonstrated that in humans, ZPB also induces acrosomal exocytosis through a Gi independent pathway.

Key words: acrosome reaction/recombinant human zona pellucida glycoproteins/spermatozoa

Financial support for these studies was provided under the Indo–US Joint Program on Contraceptive and Reproductive Health Research by Department of Biotechnology, Government of India under a Co-operative Agreement. Sanchita Chakravarty is recipient of Senior Research Fellowship, Council of Scientific and Industrial Research, Government of India. The views expressed by the authors do not necessarily reflect the views of the funding agencies.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
P. Bansal, K. Chakrabarti, and S. K. Gupta
Functional Activity of Human ZP3 Primary Sperm Receptor Resides Toward Its C-Terminus
Biol Reprod, July 1, 2009; 81(1): 7 - 15.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
P. C.N. Chiu, B. S.T. Wong, M.-K. Chung, K. K.W. Lam, R. T.K. Pang, K.-F. Lee, S.B. Sumitro, S.K. Gupta, and W. S.B. Yeung
Effects of Native Human Zona Pellucida Glycoproteins 3 and 4 on Acrosome Reaction and Zona Pellucida Binding of Human Spermatozoa
Biol Reprod, November 1, 2008; 79(5): 869 - 877.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
P. C.N. Chiu, B. S.T. Wong, C.L. Lee, R. T.K. Pang, K.-F. Lee, S.B. Sumitro, S.K. Gupta, and W. S.B. Yeung
Native human zona pellucida glycoproteins: purification and binding properties
Hum. Reprod., June 1, 2008; 23(6): 1385 - 1393.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
G. Goudet, S. Mugnier, I. Callebaut, and P. Monget
Phylogenetic Analysis and Identification of Pseudogenes Reveal a Progressive Loss of Zona Pellucida Genes During Evolution of Vertebrates
Biol Reprod, May 1, 2008; 78(5): 796 - 806.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
Y. Ni, K. Li, W. Xu, L. Song, K. Yao, X. Zhang, H. Huang, Y. Zhang, and Q.-X. Shi
Acrosome Reaction Induced by Recombinant Human Zona Pellucida 3 Peptides rhuZP3a22~176 and rhuZP3b177~348 and Their Mechanism
J Androl, May 1, 2007; 28(3): 381 - 388.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
A. Hasegawa, N. Kanazawa, H. Sawai, S. Komori, and K. Koyama
Pig zona pellucida 2 (pZP2) protein does not participate in zona pellucida formation in transgenic mice.
Reproduction, September 1, 2006; 132(3): 455 - 464.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
P. Caballero-Campo, M. Chirinos, X. J. Fan, M. E. Gonzalez-Gonzalez, M. Galicia-Chavarria, F. Larrea, and G. L. Gerton
Biological Effects of Recombinant Human Zona Pellucida Proteins on Sperm Function
Biol Reprod, April 1, 2006; 74(4): 760 - 768.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.