Skip Navigation


Mol. Hum. Reprod. Advance Access originally published online on August 26, 2005
Molecular Human Reproduction 2005 11(8):537-542; doi:10.1093/molehr/gah203
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
11/8/537    most recent
gah203v2
gah203v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (2)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Haimov-Kochman, R.
Right arrow Articles by Hurwitz, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Haimov-Kochman, R.
Right arrow Articles by Hurwitz, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org

Expression and regulation of Sprouty-2 in the granulosa-lutein cells of the corpus luteum

Ronit Haimov-Kochman1, Amir Ravhon1, Diana Prus2, Caryn Greenfield1, Zvesdana Finci-Yeheskel1, Debra S.Goldman-Wohl1, Shira Natanson-Yaron1, Reuven Reich3, Simcha Yagel1 and Arye Hurwitz1,4

1Department of Obstetrics and Gynecology, 2Department of Pathology, Hadassah University Medical Center and 3Department of Clinical Pharmacology, The Hebrew University, Jerusalem, Israel

4 To whom correspondence should be addressed at: Department of Obstetrics and Gynecology, The Division of Reproductive Endocrinology and Infertility, Hadassah Hebrew University Medical Center, Mt. Scopus, P.O.Box 24035, Jerusalem 91240, Israel. E-mail: hurwitz{at}cc.huji.ac.il

Growth factor signalling has important modulatory roles in the process of human follicular growth, oocyte maturation and corpus luteum (CL) formation. Recently, Sprouty-2, an inhibitor of receptor tyrosine kinase (RTK) signalling pathway was advocated as a marker of oocyte competence in the bovine ovary. We sought to study Sprouty-2 expression and regulation in the human ovary. RT–PCR was used to detect Sprouty-2 mRNA in human granulosa-lutein cells (GLC) collected from follicular aspiration of IVF patients. The effect of epidermal and fibroblast growth factors (EGF and FGF) on Sprouty-2 mRNA expression in GLC was studied using quantitative real-time PCR. Immunohistochemistry was performed on cultured GLC, human CL and stimulated rat ovary sections. Sprouty-2 mRNA was expressed in human GLC. EGF and basic FGF, but not FGF4 and FGF10, increased Sprouty-2 mRNA expression in GLC. The Sprouty protein was localized to GLC of early and late human CL but not to the theca cell layer. Immunostaining of developing rat CL confirmed the temporal and spatial expression of Sprouty in humans. The detection of Sprouty-2 mRNA and protein in human GLC may suggest a role for Sprouty-2 during the final stages of follicle maturation and CL formation.

Key words: corpus luteum/follicle/granulosa/ovary/Sprouty


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
K. Sugiura, Y.-Q. Su, Q. Li, K. Wigglesworth, M. M. Matzuk, and J. J. Eppig
Fibroblast Growth Factors and Epidermal Growth Factor Cooperate with Oocyte-Derived Members of the TGFbeta Superfamily to Regulate Spry2 mRNA Levels in Mouse Cumulus Cells
Biol Reprod, November 1, 2009; 81(5): 833 - 841.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Bundschu, U. Walter, and K. Schuh
The VASP-Spred-Sprouty Domain Puzzle
J. Biol. Chem., December 1, 2006; 281(48): 36477 - 36481.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.