Mol. Hum. Reprod. Advance Access originally published online on August 3, 2006
Molecular Human Reproduction 2006 12(10):619-624; doi:10.1093/molehr/gal067
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Interleukin-1-induced NF-
B recruitment to the oxytocin receptor gene inhibits RNA polymerase II–promoter interactions in cultured human myometrial cells
1Department of Obstetrics and Gynecology, 2Sealy Center for Molecular Science, 3Department of Internal Medicine, University of Texas Medical Branch, Galveston, TX, USA
4 To whom correspondence should be addressed at: Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, TX 77555-1062, USA. E-mail: msoloff{at}utmb.edu
The myometrial oxytocin receptor (OTR) is highly regulated during pregnancy, reaching maximal concentrations near term. These levels are then abruptly reduced in advanced labour and the post-partum period. Our goal was to examine the molecular basis for this reduction, using chromatin immunoprecipitation (ChIP). Interleukin-1
(IL1A) treatment of cultured human myometrial cells has previously been shown to reduce steady-state levels of OTR mRNA. We show further that IL1A reduced RNA polymerase II cross-linking to the otr promoter, as reflective of transcriptional inhibition. IL1A also increased the recruitment of nuclear factor 
B (NF-B) to a site 955 bp upstream from the transcriptional start site. Inhibition of NF-B activation negated the effects of IL1A on polymerase II dissociation, indicating a causal relationship, at least in part, between recruitment of NF-B and detachment of polymerase from the otherwise constitutively active otr promoter. IL1A treatment also resulted in increased histone H4 acetylation in the otr promoter region. Whereas NF-B recruitment and histone acetylation are generally associated with activation of gene expression, our findings show that both processes can be involved in dissociation of RNA polymerase II from an active promoter. The results of these studies suggest that the elevation of IL1 in the myometrium occurring at the end of pregnancy initiates the process of down-regulation of OTRs in advanced labour, resulting in the desensitization of the myometrium to elevated levels of OT in the blood during lactation.
Key words:
chromatin immunoprecipitation/interleukin-1/myometrial cell/NF-B/oxytocin receptor
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