Skip Navigation


Mol. Hum. Reprod. Advance Access originally published online on August 25, 2006
Molecular Human Reproduction 2006 12(10):633-641; doi:10.1093/molehr/gal072
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
12/10/633    most recent
gal072v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (1)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Meraz-Cruz, N.
Right arrow Articles by Vadillo-Ortega, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Meraz-Cruz, N.
Right arrow Articles by Vadillo-Ortega, F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Identification of a calcium-dependent matrix metalloproteinase complex in rat chorioallantoid membranes during labour

N. Meraz-Cruz1, A. Ortega2,3, G. Estrada-Gutierrez1, A. Flores1, A. Espejel1, C. Hernandez-Guerrero4 and F. Vadillo-Ortega1,5

1Direccion de Investigacion, 2Departamento de Bioquimica, Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes, 3Departamento de Bioquimica, Facultad de Medicina, Universidad Nacional Autonoma de Mexico, and 4Departamento de Microscopia Electronica, Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes, Mexico City, Mexico

5 To whom correspondence should be addressed at: Instituto Nacional de Perinatologia Isidro Espinosa de los Reyes, Montes Urales 800, Lomas de Virreyes, Mexico, D.F. 11000, Mexico. E-mail: fvadillo{at}servidor.inper.edu.mx

The induction of the expression of matrix metalloproteinases (MMPs) and their extracellular activation are key processes in connective tissue degradation in the chorioallantoid membrane during rat labour. However, the regulatory mechanisms remain largely unknown. Here, we report the identification of a calcium-dependent high molecular weight complex composed of MMP-9, MMP-3, MMP-2, tissue inhibitor of metalloproteinase 1 (TIMP-1) and TIMP-2, identified by zymography and western blotting. Molecular sieve chromatography confirmed the presence of a complex of MMPs and TIMPs with an exclusion volume >670 kDa. Differential scanning calorimetry of the complex confirmed the existence of a macromolecular complex that unfolds with a broad transition; it is denatured over a wide range of temperatures and has a Tm of 72°C in the presence of Ca2+. When denatured in the absence of Ca2+, there were at least eight transitions with Tms that corresponded to pro-MMP-9, MMP-9, pro-MMP-3, MMP-3, pro-MMP-2, MMP-2, TIMP-1 and TIMP-2. Co-localization of the same molecular components was demonstrated by confocal microscopy using cell-depleted chorioallantoid membranes. The assembly and disassembly of the complex can be reproduced at physiological concentrations of Ca2+. This complex provides a potential mechanism for the enzymatic regulation of MMPs, which may participate in connective tissue degradation leading to the rupture of the fetal membranes during labour.

Key words: chorioallantoid/extracellular matrix/matrix metalloproteinases/TIMP


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol Hum ReprodHome page
G. Garcia-Lopez, F. Vadillo-Ortega, H. Merchant-Larios, R. Maida-Claros, M. Osorio, D. Soriano-Becerril, H. Flores-Herrera, J. Beltran-Montoya, Y. Garfias-Becerra, and V. Zaga-Clavellina
Evidence of in vitro differential secretion of 72 and 92 kDa type IV collagenases after selective exposure to lipopolysaccharide in human fetal membranes
Mol. Hum. Reprod., June 1, 2007; 13(6): 409 - 418.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.