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Mol. Hum. Reprod. Advance Access originally published online on March 23, 2006
Molecular Human Reproduction 2006 12(3):169-179; doi:10.1093/molehr/gal011
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Gene expression profiling of human placentas from preeclamptic and normotensive pregnancies

S.R. Hansson1,6, Y. Chen2, J. Brodszki1, M. Chen3, E. Hernandez-Andrade1, J.M. Inman3,4, O.A. Kozhich3,4, I. Larsson1, K. Marsál1, P. Medstrand5, C.C. Xiang3 and M.J. Brownstein3

1Department of Obstetrics and Gynecology, Lund University Hospital, Lund, Sweden, 2Cancer Genetics Branch, National Human Genome Research Institute, 3Laboratory of Genetics, National Institutes of Mental Health, National Institutes of Health, Bethesda, 4SAIC Frederick, National Cancer Institute at Frederick, Fredrick, MD, USA and 5Department of Cell and Molecular Biology, Lund University, Lund, Sweden

6 To whom correspondence should be addressed at: Department of Obstetrics and Gynecology, BMC-C14, Lund University, University Hospital Lund, S-221 85 Lund, Sweden. E-mail: stefan.hansson{at}med.lu.se

The aim of this study was to investigate patterns of gene expression in placental samples from patients with preeclampsia (PE), persistent bilateral uterine artery notching (without PE), and normal controls. This study included placental tissue from nine women with PE, seven with uncomplicated pregnancies and five with bilateral uterine artery notching in Doppler velocimetry tracings. Human cDNA microarrays with 6500 transcripts/genes were used and the results verified with real-time PCR and in-situ hybridization. Multidimensional scaling method and random permutation technique demonstrated significant differences among the three groups examined. Within the 6.5K arrays, 6198 elements were unique cDNA clones representing 5952 unique UniGenes and 5695 unique LocusLinks. Multidimensional scaling plots showed 5000 genes that met our quality criteria; among these, 366 genes were significantly different in at least one comparison. Differences in three genes of interest were confirmed with real-time PCR and in-situ hybridization; acid phosphatase 5 was shown to be overexpressed in PE samples and calmodulin 2 and v-rel reticuloendotheliosis viral oncogene homolog A (RELA) were downregulated in PE and uterine artery notch placentas. In conclusion downregulation of RELA and calmodulin 2 might represent an attempt by the placenta to compensate for elevations in intracellular calcium, possibly caused by hypoxia and/or apoptosis, in both pregnancies with uterine artery notching and preeclampsia.

Key words: Doppler ultrasound/hypertension/microarray/placenta/pregnancy


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V. Sitras, R.H. Paulssen, H. Gronaas, A. Vartun, and G. Acharya
Gene expression profile in labouring and non-labouring human placenta near term
Mol. Hum. Reprod., January 1, 2008; 14(1): 61 - 65.
[Abstract] [Full Text] [PDF]


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