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Mol. Hum. Reprod. Advance Access originally published online on March 23, 2006
Molecular Human Reproduction 2006 12(5):309-319; doi:10.1093/molehr/gal034
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Decidualization and maintenance of a functional prostaglandin system in human endometrial cell lines following transformation with SV40 large T antigen

Pierre Chapdelaine1, Jihong Kang1, Sofia Boucher-Kovalik1, Nicolas Caron2, Jacques P. Tremblay2 and Michel A. Fortier1,3

1Unité de Recherche en Ontogénie et Reproduction and 2Unité de Recherche en Génétique Humaine, Centre de Recherche du CHUL, CHUQ, Faculté de Médecine, Université Laval, Sainte-Foy, Québec, Canada

3 To whom correspondence should be addressed at: Unité de recherche en Ontogénie et Reproduction, Centre de Recherche du CHUL, 2705, boul Laurier, Sainte-Foy, Québec, Canada G1V 4G2. E-mail: michel.a.fortier{at}crchul.ulaval.ca

Prostaglandins (PGs) are key regulators of reproductive function and associated pathologies. We have established stable endometrial stromal and epithelial cell lines with SV40 large T antigen (TAG) as a model to study PG action in the human endometrium. Two clones for each cell type were selected for rapid growth, PG production and response to interleukin-1ß (IL-1ß). The resulting stromal (HIESC) and epithelial (HIEEC) cells retain their characteristics for at least 40 population doublings (PDs). The selected clones express progesterone (PR) and estrogen receptor-{alpha} (ER-{alpha}) at both mRNA and protein levels. By contrast, with the existing known human endometrial cell lines Ishikawa and KLE, HIESC and HIEEC increase their production of PGF2{alpha} and PGE2 and cyclooxygenase (COX)-2 protein expression in response to IL-1ß. The latter cells also express the main biosynthetic enzymes involved in PG production, cytosolic phospholipase A2 (PLA2), COX-1 and COX-2, PGF synthase and PGE synthase and the corresponding EP2, EP3, EP4 and FP receptors. The selective COX-2 inhibitor NS-398 completely inhibits the increased production of PGs induced by IL-1ß in both cell types, whereas dexamethasone (DEX) exerts a stronger inhibition in HIESC than in HIEEC. The latter observation may be related to the higher expression of COX-1 measured in HIEEC. On the basis of the present characterization and previous determination of corresponding primary cell cultures, HIESC and HIEEC appear appropriate to study the contribution of PGs in the regulation of human endometrium function and associated pathologies.

Key words: IL1-B/epithelial cells/stromal cells/non steroidal anti-inflammatory drugs/dexamethasone


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