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Mol. Hum. Reprod. Advance Access originally published online on October 31, 2007
Molecular Human Reproduction 2007 13(12):845-851; doi:10.1093/molehr/gam075
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© The Author 2007. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Hormonal regulation of G{alpha}i2 and mPR{alpha} in immortalized human oviductal cell line OE-E6/E7

Kati S. Mönkkönen1,2, Reza Aflatoonian2, Kai-Fai Lee3, William S.B. Yeung3, Sai-Wah Tsao4, Jarmo T. Laitinen5 and Alireza Fazeli2,6

1Department of Pharmacology and Toxicology, University of Kuopio, FIN-70211 Kuopio, Finland 2Academic Unit of Reproductive and Developmental Medicine, The University of Sheffield, Level 4, Jessop Wing, Tree Root Walk, Sheffield S10 2SF, UK 3Department of Obstetrics and Gynaecology, The University of Hong Kong, Pokfulam, Hong Kong 4Department of Anatomy, The University of Hong Kong, Pokfulam, Hong Kong 5Institute of Biomedicine, Department of Physiology, University of Kuopio, FIN-70211 Kuopio, Finland

6 Correspondence address. Tel: +44-114-226-8195; Fax: +44-114-226-8538; E-mail: a.fazeli{at}sheffield.ac.uk

Heterotrimeric G proteins play a key role in membrane-mediated cell-signalling and hormonal regulation. Our earlier studies gave evidence of G protein subunit G{alpha}i2 being under hormonal regulation in human in vivo. In this study, we used immortalized human oviduct epithelial cell line OE-E6/E7 as a model to study the hormonal regulation of G{alpha}i2. We aimed at clarifying whether estradiol or progesterone could individually regulate the expression of G{alpha}i2 and its potential signalling partners. Furthermore, we aimed to investigate which sex hormone receptors could potentially mediate the gene regulation in OE-E6/E7 cell line. OE-E6/E7 cells were cultured for 5 days with different concentrations of estradiol or progesterone. Quantitative real-time polymerase chain reaction (Q-PCR) was performed using cDNA of the hormone-treated cells to reveal any changes in gene expression. The presence of potential receptor targets in these cells was studied using PCR. Our data clearly showed that low concentrations of estradiol up-regulated the expression of G{alpha}i2 gene and down-regulated the expression of membrane progesterone receptor mPR{alpha} gene in OE-E6/E7 cell line. Progesterone had no significant effect on G{alpha}i2 gene expression, but it caused up-regulation of mPR{alpha} gene expression. In conclusion, it appears that sex hormones regulate the expression of G{alpha}i2 and mPR{alpha} genes in a reverse manner in OE-E6/E7 cells. Our results suggest that estrogen receptor ERβ mediates the regulatory effects of estradiol in these cells.

Key words: estradiol/fallopian tube/G protein/progesterone/regulation

Submitted on September 2, 2007; accepted on September 24, 2007.


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