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Mol. Hum. Reprod. Advance Access originally published online on December 11, 2006
Molecular Human Reproduction 2007 13(2):111-116; doi:10.1093/molehr/gal105
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Status of p53 in first-trimester cytotrophoblastic cells

M. Cohen, A. Meisser, L. Haenggeli, I. Irminger-Finger and P. Bischof1

Department of Obstetrics and Gynaecology, Maternity, Laboratory of Hormonology, University of Geneva, Geneva, Switzerland

1 To whom correspondence should be addressed at: Laboratoire d’Hormonologie, 32 Boulevard de la Cluse, 1211 Genève 14, Switzerland. E-mail: paul.bischof{at}hcuge.ch


  Abstract

p53 has been called the cellular gatekeeper of the genome because it can induce cell-cycle arrest in G1, apoptosis or affect DNA replication in response to DNA damage. As p53 has been observed in first-trimester cytotrophoblastic cells (CTB), but its expression in normal cells is generally not detectable because of its short half-life, p53 could play an important role in cellular differentiation and/or in the control of the invasion of trophoblastic cells; therefore, p53 status was investigated in these cells. Using different antibodies recognizing different epitopes of p53 protein, abundant p53 expression was observed both in nuclear and in cytoplasmic compartments of first-trimester CTB. Whereas p53 was detected in the nuclei of few trophoblastic cells with an antibody recognizing the N-terminal epitope of the protein, high expression level of p53 in the cytoplasm of CTB was detected with an antibody recognizing the middle part of p53. The lack of immunoreactivity of p53 with antibodies recognizing the epitopes located at the N-terminus of p53 and the high level of p53 protein observed in the cytoplasm of CTB suggest that the N-terminus of p53 is involved in the formation of complexes. These cytoplasmic complexes were detected under non-reducing conditions in western blot analysis and had apparent molecular weights (MW) of 195, 167 or 125 kDa. These complexes could prolong the half-life of p53 in the cytoplasm of CTBs. By contrast, in the nuclei of CTBs, p53 seems to be present as a tetramer.

Key words: complex/CTB/cytoplasm/nucleus/p53


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M. Cohen, C. Wuillemin, O. Irion, and P. Bischof
Regulation of MMP-9 by p53 in first trimester cytotrophoblastic cells
Hum. Reprod., July 16, 2008; (2008) den264v1.
[Abstract] [Full Text] [PDF]


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