Mol. Hum. Reprod. Advance Access originally published online on December 18, 2006
Molecular Human Reproduction 2007 13(2):135-140; doi:10.1093/molehr/gal106
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Interleukin-10 gene promoter polymorphisms and their protein production in peritoneal fluid in patients with endometriosis
Department of Gynecology, Women’s Hospital, Zhejiang University School of Medicine, Hangzhou Zhejiang, P. R. China
1 To whom correspondence should be addressed at: Department of Gynecology, Women’s Hospital, Zhejiang University School of Medicine, Hangzhou Zhejiang, 310006, P. R. China. E-mail: xinmei6{at}yahoo.com
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Abstract |
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Although associations of interleukin-10 (IL-10) gene promoter polymorphisms and their protein production with endometriosis risk have been reported, the correlations remain controversial. The objective of this study was to determine IL-10 gene promoter polymorphisms at -1082, -819 and -592 sites and their protein production in peritoneal fluid (PF) in patients with and without endometriosis. IL-10 gene promoter polymorphisms at -1082 site were detected by amplification refractory mutation system (ARMS)–PCR and that at -819 and -592 sites was genotyped by restriction fragment length polymorphism (RFLP)-PCR. Protein levels of IL-10 in PF were measured by enzyme-linked immunosorbent assay (ELISA). There were no significant differences in the genotype and allele frequencies of IL-10 gene promoter polymorphisms at position -1082 between the endometriosis and the control groups. However, the frequency of -819 or -592 C alleles was significantly increased in patients with endometriosis compared with controls. The protein levels of IL-10 in PF were statistically higher in the endometriosis group than in the control group. Moreover, the polymorphisms at -1082, -819 and -592 sites were associated with protein levels of IL-10 in PF in the endometriosis group while in the control group only the polymorphisms at position -1082 correlated with protein levels. Increased frequency of -819 or -592 C allele and increased protein production of IL-10 in PF in patients with endometriosis compared with controls and correlations of polymorphisms at -819 and -592 sites with protein levels of IL-10 in PF in patients with endometriosis may suggest that polymorphisms at -819 and -592 sites and their protein production are associated with endometriosis risk.
Key words: endometriosis/interleukin-10/gene polymorphism/peritoneal fluid/protein production
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