Mol. Hum. Reprod. Advance Access originally published online on March 14, 2007
Molecular Human Reproduction 2007 13(5):343-349; doi:10.1093/molehr/gam007
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Increased expression of latent TGF-ß binding protein-1 and fibrillin-1 in human uterine leiomyomata
1 Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, CA, USA 2 Department of Gynecology and Obstetrics, Peking University, Third Hospital, Beijing, China
3 To whom correspondence should be addressed at: Department of Obstetrics and Gynecology, Stanford University, 300 Pasteur Drive, Room HH333, MC: 5317, Stanford, CA, USA. E-mail: bchen{at}stanford.edu
We compared latent TGF-ß binding protein-1 (LTBP-1) and fibrillin-1 (FBN-1) expression in leiomyomata and myometrium, correlated with leiomyomata size. We studied in vivo and in vitro effects of ovarian steroids using matched leiomyomata and myometrium samples from both phases of the menstrual cycle. Leiomyomata were divided into small (
2 cm), medium (3–5 cm) and large (
6 cm) groups. We validated LTBP-1 and FBN-1 expression using QPCR, western blot and immunohistochemistry. LTBP-1 and FBN-1 mRNA and protein expressions were higher in the medium-sized group compared with myometrium in the proliferative phase (P = 0.01; P = 0.01). FBN-1 mRNA expression was higher in the secretory phase (P = 0.01). LTBP-1 mRNA and protein expression was higher in the medium group compared with the small and large groups in the proliferative phase (P = 0.04; P = 0.04). No differences between groups were seen in FBN-1 expression in either phase. 17ß-eestradiol (E2) increased mRNA and protein expression of LTBP-1 and FBN-1 in cultured leiomyoma smooth muscle cells (LSMC) (P < 0.05). No change in FBN-1 and LTBP-1 expression was observed when cells were treated with E2 plus progesterone. Estrogen may be involved in LTBP-1 and FBN-1 expression in leiomyomata. Extracellular matrix metabolism may be different in medium-sized leiomyoma.
Key words: E2/FBN-1/leiomyomata/LTGF-ß-1/myometrium/17ß-estradiol
Submitted on November 17, 2006; resubmitted on January 12, 2007; accepted on January 22, 2007.
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