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Mol. Hum. Reprod. Advance Access originally published online on January 19, 2008
Molecular Human Reproduction 2008 14(2):97-106; doi:10.1093/molehr/gam091
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© The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Gene expression analysis of human endometrial endothelial cells exposed to op'-DDT

C. Bredhult1, L. Sahlin2 and M. Olovsson1,3

1Department of Women’s and Children’s Health, Uppsala University, SE-751 85 Uppsala, Sweden 2Department of Woman and Child Health, Karolinska Institutet, SE-171 76 Stockholm, Sweden

3 Correspondence address. E-mail: matts.olovsson{at}kbh.uu.se

The endocrine disrupting chemical o, p'-dichlorodiphenyltrichloroethane (DDT) can affect reproductive organs, tissues and cells in several species. Treatment of human endometrial endothelial cells (HEECs) with 50 µM o,p'-DDT decreased their proliferation compared with the control. Microarray analyses revealed that o,p'-DDT affected biological processes such as the cell cycle, cell division, defence response and lipid and steroid metabolism, in cellular components such as the plasma membrane and chromosomes, with molecular functions involved in signalling, receptor and cytokine activity, confirming the results of the proliferation assay. Expression of five of the most differentially expressed genes identified in the microarray analysis was verified by real-time quantitative reverse transcription polymerase chain reaction in five HEEC cultures obtained from women in the proliferative phase and in five cultures obtained from women in the secretory phase of the menstrual cycle after treatment with o,p'-DDT. The present study supports our previous findings of decreased proliferation and increased cell death in response to o,p'-DDT and may offer important clues to the mechanisms of action of o,p'-DDT.

Key words: endometrial cells/in vitro cell cultures/microarray/o,p'-DDT/reproductive tissue

Submitted on October 23, 2007; resubmitted on December 10, 2007; accepted on December 19, 2007.


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