Molecular Human Reproduction Vol. 2, NUMBER 1 pp. 40-45, 1996
© European Society of Human Reproduction and Embryology 1996
research-article |
Endocrinology and paracrinology
Interleukin-8 concentration in peritoneal fluid of patients with endometriosis and modulation of interleukin-8 expression in human mesothelial cells
1Department of Obstetrics and Gynecology 2Department of Pathology, Yale University School of Medicine 333 Cedar Street, New Haven, CT 06510, USA
To whom correspondence should be addressed at: 3To whom correspondence should be addressed
lnterleukin-8 (IL-8) is a chemoattractant and activating factor for human neutrophils and a potent angiogenic agent. The peritoneal fluid of women with endometriosis has been shown to have increased neutrophil chemotactic activity. We postulate that IL-8 may be an important modulator in the pathogenesis of endometriosis and adhesion formation. We first investigated IL-8 concentrations in the peritoneal fluid of women with or without endometriosis, then assessed peritoneal mesothelial cells as a potential source of peritoneal fluid IL-8. Northern blot analysis and enzyme-linked immunosorbent assay (ELISA) were used to investigate IL-8 mRNA and protein modulation. The mean concentration of IL-8 in samples obtained from control patients (n = 28) was 4.8 ± 0.5 pg/ml; from patients with minimal-mild endometriosis (n = 24) was 27.5 ± 2.6 pg/ml; and from patients with moderate-severe endometriosis (n = 21) was 530.2 ± 65.1 pg/ml. Confluent mesothelial cells were incubated with human recombinant IL-1a (0.01–100 IU/ml) or tumour necrosis factor (TNF)-a (0.01 to 100 ng/ml) for 2–24 h. IL-8 mRNA was detectable in non-treated cells, however both IL-1a and TNF-
induced higher amounts of IL-8 mRNA in a dose- and time-dependent manner. Non-treated mesothelial cells in culture also produced and secreted IL-8 protein quantified by ELISA, but again higher concentrations were induced by IL-1 and TNF- treatment. In conclusion, we found that IL-8 concentrations were elevated in peritoneal fluids from women with endometriosis. Cultured mesothelial cells expressed cytokine-inducible IL-8 mRNA and secreted IL-8 protein. The regulated expression of this angiogenic factor may play a role in pathogenesis of endometriosis.
endometriosis/interleukin-8/mesothelial cells/peritoneal fluid
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