Molecular Human Reproduction Vol. 2, NUMBER 2 pp. 123-129, 1996
© European Society of Human Reproduction and Embryology 1996
research-article |
Nuclear retinoid receptor expression in normal human endometrium throughout the menstrual cycle
1Department of Obstetrics and Gynaecology, University of Newcastle Newcastle upon Tyne, NE2 4HH, UK 2Department of Medicine, The Medical School, University of Newcastle Newcastle upon Tyne, NE2 4HH, UK 3Department of Obstetrics and Gynaecology, University of Southampton, Princess Anne Hospital Southampton SO9 4HA, UK 4Current address: Department of Obstetrics and Gynaecology, University of Cambridge, Rosie Maternity Hospital Cambridge CB2 2SW, UK
To whom correspondence should be addressed at: 5To whom correspondence should be addressed
Previous work has shown that retinoic acid receptors (RARs) and retinoid X receptors (RXRs) are expressed in human endometrial epithelial and stromal cells. These nuclear receptors mediate the biological effects of retinoic acid, a vitamin A derivative which may have an important, though poorly characterized role in the functional differentiation of secretory eprthelia. The aim of this study was to find out whether the expression of RAR and RXR mRNA in endometrial epithelial and stromal cells varies in relation to the menstrual cycle. The expression of RARs and RXRs was investigated by Northern blotting and, for stromal cells, there were no differences in expression of RAR-
RAR-ß RAR-
and RXR- between the prolrferative and secretory phases of the menstrual cycle. Similarly, for epithelial tissue, there were no significant differences between the prolrferative and secretory phases with respect to the expression of RAR- RAR- and RXR-. However, RAR-ß was expressed at a 1.7-fold higher level in epithelial samples from the prolrferative phase compared to the secretory phase. Overall, the levels of expression of RAR- RAR-ß and RAR- were 1.7- to 4-fold higher in stromal cells compared to epithelial cells whereas RXR- was expressed at a similar level in both cell types. We have previously suggested that retinoic acid has a role in endometrial differentiation or function which may be reflected by cyclical changes in intracellular retinoic acid levels. These data indicate that RARs and RXRs are expressed at a similar level throughout the menstrual cycle, with the possible exception of RAR-ß implying that any menstrual cycle-related function of RARs is controlled by ligand availability rather than by changes in expression of the receptors.
human epithelium/gene expression/menstrual cycle/retinoic acid receptors/retinoid X receptors
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