Molecular Human Reproduction Vol. 2, NUMBER 2 pp. 135-138, 1996
© European Society of Human Reproduction and Embryology 1996
research-article |
The PRINS technique: potential use for rapid preimplantation embryo chromosome screening
1CNRS UPR 9008, Route de Mende, BP 5051, F-34033 Montpellier 2Cytogenetics Laboratory, St. Charles Hospital F-34295 Montpellier, France 3MRC Human Genetics Unit, Western General Hospital Crewe Road, Edinburgh EH4 2XU, UK
To whom correspondence should be addressed at: 4To whom correspondence should be addressed
The primed in-situ labelling (PRINS) method is an alternative to in-srtu hybridization for chromosomal detection based on the use of chromosome-specific oligonucleotide primers. Using this process, we have developed a simple and semi-automatic method for rapid in-situ detection of human chromosomes. The reaction was performed on a programmable temperature cycler. Specific labelling was obtained in < 2 h reaction. Double PRINS techniques were performed on six morphologically abnormal preimplantation embryos using primers specific for chromosomes 9, 16, 18, 21, X and Y. The majority of these embryos displayed chromosomal abnormalities. The present results demonstrate that PRINS may be a simple and reliable technique applicable in human preimplantation diagnosis.
human pre-embryo/in-situ labelling/oligonucleotide/preimplantation diagnosis/PRINS