Molecular Human Reproduction Vol. 2, NUMBER 2 pp. 99-104, 1996
© European Society of Human Reproduction and Embryology 1996
research-article |
Preliminary studies on the role of plasminogen activator in seminal plasma of human and rhesus monkey
1State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences 19 Zhongguancun Lu, Haidian, Beijing 100080, China 2National Research Institute for Family Planning Beijing 100081, China
To whom correspondence should be addressed at: 5To whom correspondence should be addressed
Two types of plasminogen activators (PA), tissue type (tPA) and urokinase type (uPA), were identified in the seminal plasma of both the human and the rhesus monkey. We studied the possible relationship between PA activities in the seminal plasma and the sperm counts and motility and demonstrated that: (i) PA activity in human seminal plasma from infertile patients was associated with immotile spermatozoa; (ii) the treatment of fertile men with testosterone enanthate (TE) to induce azoospermia was accompanied by an increase in seminal PA activity; (iii) when monomer T4 (isolated from multiglycosides of Tripterygium wilforddi) was administered to fertile male rhesus monkeys to induce azoospermia, PA activities in seminal plasma increased considerably; and (iv) immunocytochemistry studies showed that both uPA and PAI-1 antigens were localized on the surface of human spermatozoa, indicating that human spermatozoa were capable of binding uPA and PAI-1 through their receptors or forming a complex. These data demonstrate that seminal PA activity may be related to azoospermia, and possibly, to the fertilizing capability of spermatozoa in primates.
human/plasminogen activator/rhesus monkey/seminal plasma
3Present Address: Department of Medical Biochemistry and Biophysics, Umeå University,S-901 87, Umeå, Sweden
4Present Address: Department of Pathology, McMaster University,Ontario L8N 3Z5, Canada
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