Molecular Human Reproduction Vol. 2, NUMBER 3 pp. 169-175, 1996
© European Society of Human Reproduction and Embryology 1996
The expression of 
V, 5, ß1, and ß3 integrin chains on ejaculated human spermatozoa varies with their functional state
F.M. Fusi1,
C. Tamburini1,
F. Mangili2,
M. Montesano1,
A. Ferrai1 and
R.A. Bronson3,4
1Department of Obstetrics and Gynecology Milano, Italy
2Pathology San Raffaele Hospital, University of Milano Milano, Italy
3Department of Obstetrics and Gynecology, SUNY at Stony Brook Stony Brook, New York 11784-8091, USA
To whom correspondence should be addressed at:
4 To whom correspondence should be addressed
Evidence has been presented suggesting the involvement of integrins and their ligands in mammalian fertilization. In this study we asked whether the 
5vß1 and ß3 integrin chains, which form receptors for fibronectin and vitronectin, are present on human spermatozoa. Fresh ejaculate spermatozoa and capacitated spermatozoa, before and after a calcium ionophore (A23187)-induced acrosome reaction, were either fixed and their reaction with anti-integrin monoclonal antibodies detected by immunoperoxidase staining or studied without fixation, using cytofluorimetric scanning. Expression of specific integrin chains varied with the functional state of spermatozoa. The 5 chain was not detected on fresh living spermatozoa, but was present on capacitated spermatozoa, whether fixed or living. The pattern of fa expression on living spermatozoa paralleled that of 5. No further increase in the expression of either 5 or ß1 was observed following an ionophore-promoted acrosome reaction. In contrast, v was detected on neither fresh, living ejaculate spermatozoa, nor following capacitation (<10% reactive). The percentage of v positive cells increased substantially following ionophore exposure. Expression of 3 was similar to v, and the percentage of cells displaying ß3 correlated with the proportion of spermatozoa that had undergone an acrosome reaction, following ionophore exposure. These results indicate that the expression of integrins on spermatozoa is dynamic, varying with their functional state and that integrin receptors for fibronectin (5ß1) become apparent on the spermatozoan surface during capacitation and vitronectin (vß3) following the acrosome reaction.
acrosome reaction/capacitation/integrins/spermatozoa
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