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Molecular Human Reproduction Vol. 2, NUMBER 3 pp. 209-212, 1996
© European Society of Human Reproduction and Embryology 1996

research-article

Adaptation of the primer extension preamplification (PEP) reaction for preimplantation diagnosis: single blastomere analysis using short PEP protocols

K. Sermon1,3, W. Lissens1, H. Joris2, A.Van Steirteghem2 and I. Liebaers1

1Centre for Medical Genetics Laarbeeklaan 101, 1090 Brussels, Belgium 2Centre for Reproductive Medicine, Dutch-speaking Brussels Free University Laarbeeklaan 101, 1090 Brussels, Belgium

To whom correspondence should be addressed at: 3To whom correspondence should be addressed

Primer extension preamplification (PEP) was first described as a method for whole genome amplification, starting from a single cell, originally a spermatozoon, in order to perform genetic recombination studies. Its usefulness for preimplantation diagnosis was shown soon after; the only drawback was the length of the procedure(>14 h). We have developed a shorter PEP protocol for single human blastomeres, enabling us to examine several genetic loci of interest in human genetic diseases with a good amplification efficiency.

PEP/preimplantation diagnosis/single cell PCR/whole genome amplification


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