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Molecular Human Reproduction Vol. 2, NUMBER 4 pp. 251-257, 1996
© European Society of Human Reproduction and Embryology 1996

research-article

Endocrinology and Paracrinology

Cell shape change reveals the cyclic AMP-mediated action of follicle stimulating hormone, human chorionic gonadotrophin and vasoactive intestinal peptide in primary cultured human granulosa-lutein cells

C. Furger1, M. Pouchelet3, J.R. Zorn2 and F. Ferré1,4

1INSERM U 361 123 Boulevard de Port-Royal, 75014 Paris 2Clinique de Universitaire Baudelocque, Maternité Baudelocque 123 Boulevard de Port-Royal, 75014 Paris 3INSERM Service Cinématographie, Le Vésinet France

To whom correspondence should be addressed at: 4To whom correspondence should be addressed

Granulosa cells are known to be the site of action of various hormones and agents that regulate ovarian function. This study was conducted to evaluate the effects of gonadotrophins, vasoactive intestinal peptide (VIP), prostaglandin (PG) F2{alpha} and angiotensin II on the cyclic AMP (c-AMP) signalling transduction pathway in human granulosa-lutein cells. Exposure to agents that elevate c-AMP or mimic c-AMP action caused the cells to become rounded in a process that was rapid and reversible. We were able to demonstrate this cell rounding process in the presence of gonadotrophins and VIP, but not in the presence of PGF2{alpha} or angiotensin II. In addition, incubation of the cells with various selective phosphodiesterase (PDE) inhibitors revealed that the PDE type IV isoform, but not type III, catalyses c-AMP degradation in human granulosa-lutein cells. Alteration in c-AMP-dependent cytomorphology appears to be a convenient method to analyse the regulation of c-AMP-mediated events in the human granulosa-lutein cells.

c-AMP/gonadotrophins/human granulosa-lutein cells/phosphodiesterases/VIP


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