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Molecular Human Reproduction Vol. 2, NUMBER 4 pp. 265-272, 1996
© European Society of Human Reproduction and Embryology 1996

research-article

Preimplantation embrology

The role of ryanodine-sensitive Ca2+ stores in the Ca2+ oscillation machine of human oocytes

Mario Sousa1,4, Alberto Barros2 and Jan Tesarik3

1Laboratory of Cell Biology, Institute of Biomedical Sciences, University of Oporto, Lg. Prof. Abel Salazar 2, 4000 Porto 2Laboratory of Medical Genetics, Faculty of Medicine, University of Oporto, Lg. Prof. Hernani Monteiro, 4200 Porto, Portugal 3Department of Biochemistry and Molecular Biology, University of Granada Faculty of Sciences, Campus Universitario Fuentenueva 18071 Granada, Spain

To whom correspondence should be addressed at: 4To whom correspondence should be addressed

This study was undertaken to localize ryanodine-sensitive Ca2+ stores in human oocytes and to evaluate their role in the Ca2+ oscillations responsible for oocyte activation at fertilization. The addition of ryanodine provoked a Ca2+ discharge from stores localized throughout the ooplasm with the exception of the cortical and subcortical peripheral regions. The ryanodine-induced discharge was typically followed by a short series of Ca2+ oscillations that only involved the cytoplasmic region populated by the ryanodine-sensitive stores. In contrast, the Ca2+ oscillations induced by the thiol reagent thimerosal or by spermatozoa at fertilization were of a much longer duration and also involved ryanodine-insensitive stores. Presumably, these ryanodine-insensitive stores are sensitive to inositol 1, 4, 5-trisphosphate (InsP3). The addition of ryanodine to oocytes during ongoing thimerosal- or sperm-induced Ca2+ oscillations inhibited the oscillations. These data suggest a co-operation between the ryanodine-sensitive and ryanodine-insensitive stores in maintaining the sperm-induced Ca2+ oscillations. In this two-store oscillation model, each periodic [Ca2+]i increase is triggered by a Ca2+ discharge from the peripheral, InsP3-sensitive stores inducing Ca2+-induced Ca2+ release from the ryanodine-sensitive stores. However, the pacemaker frequency of the Ca2+ discharges from the InsP3-sensitive stores is conditioned by the actual physiological state of the ryanodine-sensitive stores.

Ca2+ oscillations/Ca2+ stores/fertilization/ryanodine/thimerosal


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