Molecular Human Reproduction Vol. 2, NUMBER 5 pp. 307-315, 1996
© European Society of Human Reproduction and Embryology 1996
research-article |
Cell Proliferation and apoptosis: Detection of apoptosis in human endometriotic tissues
1Department of Obstetrics and Gynecology, Nagoya University School of Medicine 65 Tsurumai-cho, Showa-ku Nagoya 466 2Division of Pathology, Clinical Laboratory, Nagoya University Hospital Nagoya 466 3Department of Obstetrics and Gynecology, Ogaki City Hospital Ogaki 503, Japan 4Current address: Department of Obstetrics and Gynecology, Tokai Civil Hospital Tokai 477, Japan 5Current address: Department of Obstetrics and Gynecology, Handa City Hospital Handa 475, Japan
To whom correspondence should be addressed at: 6whom correspondence should be addressed
To clarify whether apoptosis is involved in endometriosis, we obtained eutopic endometrial tissues along with endometriotic tissues from the uterus (adenomyosis) (n = 12) and from the ovary (n = 12) from patients undergoing gynaecological surgery. Apoptosis-induced DNA fragmentation was detected by the TdT-mediated dUTP-biotin nick-end labelling method, and immunostaining with a monoclonal antibody against the Fas, Ley or B-cell leukaemia/lymphoma-2 (bcl-2) was also performed using the same tissue section. Analysis showed that apoptosis was occurring in all the samples of ovarian endometriotic tissue but in only two of the 12 adenomyotic and in five of the 24 eutopic endometrial tissue samples. In none of these cases was apoptosis correlated with phases of the menstrual cycle. The expression of bcl-2 in the eutopic endometrial and adenomyotic tissues was limited to the proliferative phase, and was observed in only one of the 12 cases of ovarian endometriosis. Fas and Ley were expressed randomly across a wide range in both the eutopic and ectopic endometrial tissues. These results suggest that the features of ovarian endometriosis are different from those of adenomyosis and eutopic endometrium in terms of the involvement of apoptosis. In addition, the regulatory mechanism involved in ovarian endometriosis may differ from that in other endometrial cells.
adenomyosis/apoptosis/bcl-2/DNA fragmentation/endometriosis
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