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Molecular Human Reproduction Vol. 2, NUMBER 5 pp. 335-339, 1996
© European Society of Human Reproduction and Embryology 1996

research-article

Integrins and adhesion molecules: Low expression of adhesion molecules and matrix proteins in patients showing poor penetration in zona-free hamster oocytes

R. Henkel1,4, J. Schaller2, H.J. Glander3 and W.B. Schill1

1Department of Dermatology and Andrology, Justus Liebig University Giessen Gaffkcystraße 14, 35385 Giessen 2Department of Dermatology, St. Barbara Hospital Barbara Straße 67, 47141 Duisburg 3Department of Dermatology, Andrology Unit, University of Leipzig Liebigstraße 21, 04103 Leipzig, Germany

To whom correspondence should be addressed at: 4To whom correspondence should be addressed

The expression of adhesion molecules and matrix proteins by human spermatozoa as well as their binding to and penetration of zona-free hamster eggs were investigated in 17 patients by means of flow cytometry. Both binding and penetration of hamster oocytes and expression of {alpha}-and ß-chains of ß1,ß3 and ß4 integrins were determined before and after induction of the acrosome reaction. The expression of the integrin ligands, fibronectin and laminin were also analysed. Significant differences in the expression of very late antigen (VLA) integrins, VLA{alpha}4-chain (CD49d) the classical fibronectin receptor VLA{alpha}5-chain (CD49e), leukocyte function-associated molecule-3 (LFA-3;CD5B) and fibronectin were observed between patients showing good (>10%) or poor (<10%) penetration in the sperm penetration assay (P = 0.0068). It is concluded that these adhesion molecules are intimately involved in the sperm-oolemma interaction. Since no differences were observed in either spermiogram parameters or the percentages of acrosome-reacted spermatozoa in the two groups, sperm-oolemma binding and penetration should be regarded as discrete parameters of sperm function.

adhesion molecules/fertilizing capability of human spermatozoa/matrix proteins/oolemma/sperm penetration assay


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