Molecular Human Reproduction, Vol 3, 69-75, Copyright © 1997 by Oxford University Press
JF Telfer, GA Irvine, G Kohnen, S Campbell and IT Cameron
Immunocytochemistry was used to localize endothelial (eNOS) and inducible
(iNOS) nitric oxide synthase in human uterine tissues collected at various
stages of the menstrual cycle, after exposure to exogenous progestagens,
and in early pregnancy. Endothelial NOS-like immunoreactivity was detected
in all specimens in endothelial cells lining blood vessels in the
myometrium and endometrium, and in endometrial glandular epithelial cells.
Inducible NOS-like immunoreactivity was also demonstrated in glandular
epithelial cells. For both eNOS and iNOS there was considerable variation
in the intensity of epithelial cell staining between samples, which was not
related to the stage of the menstrual cycle at which the tissue was
collected. Messenger RNA for eNOS and iNOS was detected by reverse
transcription-polymerase chain reaction (RT-PCR) using total RNA purified
from isolated endometrial gland fragments. Immunoreactivity for eNOS and
iNOS was not present in endometrial stroma throughout the menstrual cycle,
but iNOS-like immunoreactivity was seen in decidualized stromal cells both
following treatment with exogenous progestagen (intrauterine L-norgestrel)
and in tissues obtained in the first trimester of pregnancy. The detection
of protein and mRNA for eNOS and iNOS in normal human endometrium suggests
that NO may play a role in the local control of endometrial function.
JOURNAL ARTICLE
Expression of endothelial and inducible nitric oxide synthase in non- pregnant and decidualized human endometrium
Department of Obstetrics and Gynaecology, University of Glasgow, UK.
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