Molecular Human Reproduction, Vol 3, 839-846, Copyright © 1997 by Oxford University Press
S Benoff, IR Hurley, FS Mandel, T Paine, A Jacob, GW Cooper and A Hershlag
A predictive test for determining whether motile populations of human
spermatozoa will fertilize eggs in vitro has been an elusive goal of
clinical research. We have developed an assay for the ability of motile
human spermatozoa to bind fluorescein isothiocyanate-labelled mannosylated
bovine serum albumin (Man-FITC-BSA) as a test for the presence of sperm
surface receptors (lectins) for mannose ligands. Mannosylated ligands are
present on the human zona pellucida and are involved in the
species-specific binding of human spermatozoa to the zona pellucida. We now
demonstrate in prospective blinded analysis that the fractional increase in
acrosome loss following a mannose ligand challenge is highly correlated
with the rate of fertilization in vitro. Using an incremental increase of
acrosome exocytosis of >0.1 as a threshold to predict which specimens
will yield normal fertilization, the assay has a sensitivity of 97.8%, a
specificity of 83.3%, a positive predictive value of 95.7% and a negative
predictive value of 90.7%. These data indicate that testing for a
mannose-induced acrosome reaction may be useful in assessment of sperm
function prior to in- vitro fertilization in order to assign males to
conventional insemination or intracytoplasmic sperm injection protocols.
JOURNAL ARTICLE
Use of mannose ligands in IVF screens to mimic zona pellucida-induced acrosome reactions and predict fertilization success
Department of Obstetrics and Gynecology, North Shore University Hospital-New York University School of Medicine, Manhasset 11030, USA.
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