Molecular Human Reproduction, Vol 3, 1045-1051, Copyright © 1997 by Oxford University Press
CH Yeung, TG Cooper and E Nieschlag
CD52 is a glycosylphosphatidylinositol-anchored glycoprotein secreted by
the epididymis where it is incorporated into sperm membranes. The antigen
is common to spermatozoa and lymphocytes, and its role is not known.
Quantitative analysis using immunostaining with the monoclonal antibody
CAMPATH-1G and flow cytometry indicated positive signals from approximately
80% of viable, washed ejaculated spermatozoa. Staining intensity increased
after capacitation overnight, and decreased after short incubation with
high density lipoprotein. After incubation of Percoll-washed spermatozoa
with CAMPATH-1G, motility was reduced from 83 to 74% after 5 min and from
73 to 52% after 3.5 h. Swimming velocities were reduced by approximately
30% and linearity by 15% in 5 min, but no further decreases were observed
over 3.5 h. After 20 min incubation with the antibody, cell viability was
decreased by 10 and 20% in freshly washed and capacitated spermatozoa
respectively. Comparison of fertile and infertile patients revealed no
difference in the percentages of immunostained spermatozoa or in staining
intensity and there were no differences in sperm labelling between
normozoospermia and teratozoospermia. Compared with normozoospermia,
percentages of stained spermatozoa were lower by 20 and 27% in
asthenozoospermia and oligozoospermia respectively, whereas staining
intensity in asthenozoospermia was less than half that in oligozoospermia.
The correlation of percentages of stained spermatozoa with percentages of
motile cells suggests the involvement of epididymal CD52 in the maturation
of sperm function.
JOURNAL ARTICLE
Human epididymal secreted protein CD52 on ejaculated spermatozoa: correlations with semen characteristics and the effect of its antibody
Institute of Reproductive Medicine of the University, Munster, Germany.
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