Molecular Human Reproduction, Vol 3, 1053-1060, Copyright © 1997 by Oxford University Press
CP Tipler, SP Hutchon, K Hendil, K Tanaka, S Fishel and RJ Mayer
We purified by fractionation on 10-40% glycerol gradients, 26S proteasomes
from normal human spermatozoa. These proteasomes, which participate in the
ATP-dependent degradation of ubiquitinated proteins, share a similar
sedimentation coefficient to those purified from other human tissues.
Fluorogenic peptide assays reveal they have chymotrypsin, trypsin and
peptidyl-glutamyl-like peptide hydrolysing activities; the chymotrypsin
activity is ablated by the specific 26S proteasome inhibitor MG132.
Confirmation that these large proteases are 26S proteasomes is provided by
detection of the 20S proteasome subunits HC2, XAPC7, RN3 and Z and
regulatory ATPases MSS1, TBP1, SUG1 and SUG2 by Western analyses with
monoclonal antisera. These antigens are found only in the gradient
fractions enriched in proteolytic activities. We have also shown that,
although mature spermatozoa from mice have considerably reduced amounts of
a ubiquitin-conjugating enzyme (E2) and ubiquitin-protein conjugates in
comparison with less mature germ cells, they retain relatively high values
of 26S proteasome activity. This suggests that proteasomes may have further
roles to play in normal sperm physiology.
JOURNAL ARTICLE
Purification and characterization of 26S proteasomes from human and mouse spermatozoa
Department of Biochemistry, University of Nottingham Medical School, Queen's Medical Centre, UK.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  E. S. Diaz, M. Kong, and P. Morales Effect of fibronectin on proteasome activity, acrosome reaction, tyrosine phosphorylation and intracellular calcium concentrations of human sperm Hum. Reprod., May 1, 2007; 22(5): 1420 - 1430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Jones Sperm Survival Versus Degradation in the Mammalian Epididymis: A Hypothesis Biol Reprod, November 1, 2004; 71(5): 1405 - 1411. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Dong, W. Chen, A. Welford, and A. Wandinger-Ness The Proteasome {alpha}-Subunit XAPC7 Interacts Specifically with Rab7 and Late Endosomes J. Biol. Chem., May 14, 2004; 279(20): 21334 - 21342. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Escalier New Insights into the Assembly of the Periaxonemal Structures in Mammalian Spermatozoa Biol Reprod, August 1, 2003; 69(2): 373 - 378. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Morales, M. Kong, E. Pizarro, and C. Pasten Participation of the sperm proteasome in human fertilization Hum. Reprod., May 1, 2003; 18(5): 1010 - 1017. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Bohring, E. Krause, B. Habermann, and W. Krause Isolation and identification of sperm membrane antigens recognized by antisperm antibodies, and their possible role in immunological infertility disease Mol. Hum. Reprod., February 1, 2001; 7(2): 113 - 118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Sutovsky, R. D. Moreno, J. Ramalho-Santos, T. Dominko, C. Simerly, and G. Schatten Ubiquitinated Sperm Mitochondria, Selective Proteolysis, and the Regulation of Mitochondrial Inheritance in Mammalian Embryos Biol Reprod, August 1, 2000; 63(2): 582 - 590. [Abstract] [Full Text]
|
|
|
|
|
|
C. Wojcik, M. Benchaib, J. Lornage, J.C. Czyba, and J.F. Guerin Localization of proteasomes in human oocytes and preimplantation embryos Mol. Hum. Reprod., April 1, 2000; 6(4): 331 - 336. [Abstract] [Full Text] [PDF]
|
|