Molecular Human Reproduction, Vol 3, 1101-1105, Copyright © 1997 by Oxford University Press
M Sillem, S Prifti, B Monga, P Buvari, U Shamia and B Runnebaum
Extracellular matrix degradation by secreted proteases, e.g. plasmin, is
essential for endometrial functions such as blastocyst implantation and
menstruation. We investigated whether the expression of plasmin(ogen)
activating or inhibiting factors in endometrial cells from women with
endometriosis was different from women without the disease. Endometrial
biopsies were obtained from 10 patients with and 16 women without
endometriosis. Cells were cultured in Dulbecco's modified Eagle's medium
(DMEM)/F12 supplemented with diethylstilboestrol (10(-10) M) alone or
combined with promegestone (5 x 10(-8) or 5 x 10(-6) M). Urokinase
plasminogen activator (uPA), plasminogen activator inhibitor (PAI)-1 and
-2, and soluble uPA receptor (suPA-R) concentrations were assayed by
enzyme-linked immunosorbent assay (ELISA) in the conditioned media. uPA and
PAI-2 concentrations were not influenced by steroid treatment and did not
differ between women with and without endometriosis, whereas PAI-1 was
significantly up-regulated by promegestone in both groups. In contrast,
suPA-R expression was not influenced by steroid treatment but was
significantly higher in cells from endometriosis patients. This is the
first report on suPA-R secretion in endometrial cells and the results
indicate an altered activation of plasmin(ogen) in endometrium from women
with endometriosis that could lead to a higher proteolytic potential of
retrogradely menstruated endometrial fragments with consecutive development
of endometriotic foci.
JOURNAL ARTICLE
Soluble urokinase-type plasminogen activator receptor is over-expressed in uterine endometrium from women with endometriosis
Department of Obstetrics and Gynaecology, Ruprecht-Karls-Universitat, Heidelberg, Germany.
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