Molecular Human Reproduction, Vol 3, 275-283, Copyright © 1997 by Oxford University Press
M Thie, P Herter, H Pommerenke, F Durr, F Sieckmann, B Nebe, J Rychly and HW Denker
Adhesiveness of the apical (free) plasma membrane of uterine epithelial
cells for trophoblast is essential for the process of human embryo
implantation. As epithelial cells are normally repellent, i.e. apically
non-adhesive, we argue that a remodelling of the epithelial organization
from a polarized to a non-polarized phenotype might prepare the apical pole
for cell-cell adhesion during the so-called receptive phase. To identify
details of apical adhesiveness we examined human epithelial RL95-2 cells
(RL cells) which, in contrast to other cell lines, allow trophoblast to
adhere to their apical plasma membrane. To determine whether the
cytoskeletal structure is functionally critical for adhesiveness for
trophoblast, RL cells were treated with actin depolymerizing cytochalasin
D, i.e. 0.4 microM for 120 min. Changes in adhesiveness for trophoblast
were monitored with a centrifugal force-based adhesion assay. Moreover,
ultrastructural features, organization of the actin network and expression
of integrins, i.e. alpha 6, beta 1, beta 4, were studied using electron
microscopy, confocal laser scanning microscopy and cell surface
immunogold-labelling techniques. Changes in transmission of mechanical
signals via integrins into uterine cells were examined using a magnetic
drag force device, thereby monitoring intracellular calcium responses. The
results suggest that adhesiveness of the free surface of RL cells for human
trophoblast requires an intact but non-polarized actin cytoskeleton,
apically localized integrins linked to actin, and calcium signalling
originating at the free surface.
JOURNAL ARTICLE
Adhesiveness of the free surface of a human endometrial monolayer for trophoblast as related to actin cytoskeleton
Institute of Anatomy, University of Essen Medical School, Germany.
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