Molecular Human Reproduction, Vol 3, 485-491, Copyright © 1997 by Oxford University Press
TL Purcell, IA Buhimschi, R Given, K Chwalisz and RE Garfield
The aim of this study was to investigate the expression and distribution
patterns of the inducible isoform of nitric oxide synthase (iNOS) in rat
placenta during gestation and term labour. The expression of iNOS isoform
was assessed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis
(SDS-PAGE) and immunoblotting with monoclonal antibodies. Two specific
bands were detected corresponding to 135 and 124 kDa in all placenta
samples. The upper band (135 kDa) was identified as iNOS due to its
correspondence with the band obtained with mouse macrophages (positive
control). Compared with its concentrations on day 16, iNOS decreased
steadily toward the end of gestation to approximately 37% on day 20, 20% on
day 22 before labour and 12% during labour (p < 0.01). The lower band
(124 kDa) drastically increased (to almost double) from day 16 to day 18
but returned to initial values on day 22, during delivery.
Immunohistochemical staining of placentae at day 16 and 22 using rabbit
polyclonal anti-iNOS antibody revealed labelling specifically concentrated
in the trophospongial cell layer, at the fetal-maternal interface. The most
conspicuous iNOS staining was associated with islands of cells referred to
as vacuolated 'glycogen cells'. Staining was greatly decreased during
labour. The changes in placental iNOS expression suggest a 'paracrine' role
for NO in regulating uterine contractility, blood flow and
immunosuppression required for pregnancy maintenance. NO withdrawal at term
may also be involved in the initiation of labour.
JOURNAL ARTICLE
Inducible nitric oxide synthase is present in the rat placenta at the fetal-maternal interface and decreases prior to labour
Department of Obstetrics and Gynaecology, University of Texas Medical Branch, Galveston 77555-1062, USA.
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