Molecular Human Reproduction, Vol 3, 493-499, Copyright © 1997 by Oxford University Press
KA Freed, EK Moses, SP Brennecke and GE Rice
The involvement of phospholipase A2 (PLA2) enzymes in the formation of
biologically-active phospholipid metabolites by human gestational tissues
has principally been characterized by the use of enzyme activity assays.
While such assays have established the presence of functional PLA2
activity, there is a paucity of information concerning the tissue
distribution and relative contribution to net activity made by specific
PLA2 isozymes. In particular, both secretory and cytosolic isozymes may be
involved in gestational tissue phospholipid metabolism. Thus, the aim of
this study was to test the hypothesis that phospholipase A2 mRNA
transcripts encoding Type II, Type IV and cytosolic PLA2 are
tissue-specifically expressed in human amnion, choriodecidua and placenta
obtained at term. The relative expression of polyA+ mRNA encoding these
PLA2 isozymes was determined by Northern blot analysis and laser
densitometry. The data obtained confirm the tissue-specific expression of
PLA2 mRNA in human intrauterine tissues. Cytosolic PLA2 mRNA was most
abundantly expressed in amnion when compared to either choriodecidua (which
was 5-fold less than amnion; P < 0.001) or placenta (72-fold less than
amnion; P < 0.001). In contrast, the secretory PLA2 mRNA transcripts
(i.e. Type II and Type IV) were most abundantly expressed in placenta. Type
II PLA2 mRNA expression in choriodecidua and amnion was 30-fold less than
that observed in placenta (both P < 0.001). Type IV PLA2 mRNA expression
was 37-fold (P < 0.001) and 73-fold (P < 0.001) less in choriodecidua
and amnion respectively. These data support the conclusion that cytosolic
PLA2 is the principal PLA2 isozyme mediating phospholipid metabolism and
the liberation of fatty acid substrate (i.e. arachidonic acid) in term
amnion, while secretory PLA2 isozymes, and in particular, Type II PLA2 play
a major role in phospholipid metabolism in term placenta.
JOURNAL ARTICLE
Differential expression of type II, IV and cytosolic PLA2 messenger RNA in human intrauterine tissues at term
Department of Perinatal Medicine, Royal Women's Hospital, Carlton, Victoria, Australia.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  L. Gao, C. Lu, C. Xu, Y. Tao, B. Cong, and X. Ni Differential Regulation of Prostaglandin Production Mediated by Corticotropin-Releasing Hormone Receptor Type 1 and Type 2 in Cultured Human Placental Trophoblasts Endocrinology, June 1, 2008; 149(6): 2866 - 2876. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Farina, S. Billi, G. Leguizamon, C. Weissmann, T. Guadagnoli, M. L. Ribeiro, and A. M. Franchi Secretory and cytosolic phospholipase A2 activities and expression are regulated by oxytocin and estradiol during labor Reproduction, August 1, 2007; 134(2): 355 - 364. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Brant, W. Guan, P. Tithof, and R. L. Caruso Gestation Age-Related Increase in 50-kDa Rat Uterine Calcium-Independent Phospholipase A2 Expression Influences Uterine Sensitivity to Polychlorinated Biphenyl Stimulation Biol Reprod, May 1, 2006; 74(5): 874 - 880. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Varastehpour, T. Radaelli, J. Minium, H. Ortega, E. Herrera, P. Catalano, and S. Hauguel-de Mouzon Activation of Phospholipase A2 Is Associated with Generation of Placental Lipid Signals and Fetal Obesity J. Clin. Endocrinol. Metab., January 1, 2006; 91(1): 248 - 255. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Slater, S. Astle, P. R. Bennett, and S. Thornton Labour is associated with increased expression of type-IIA secretory phospholipase A2 but not type-IV cytosolic phospholipase A2 in human myometrium Mol. Hum. Reprod., November 1, 2004; 10(11): 799 - 805. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. M. Slater, S. Zervou, and S. Thornton Prostaglandins and Prostanoid Receptors in Human Pregnancy and Parturition Reproductive Sciences, May 1, 2002; 9(3): 118 - 124. [Abstract] [PDF]
|
|
|
|
|
|
B. Kleine, S. Wolfahrt, M. Lotsch, T. Gantner, and W. G. Rossmanith Expression of galanin in human placenta Mol. Hum. Reprod., April 1, 2001; 7(4): 379 - 385. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Rosenthal, E. D. Albrecht, and G. J. Pepe Developmental Maturation of Baboon Placental Trophoblast: Expression of Messenger Ribonucleic Acid and Protein Levels of Cytosolic and Secretory Phospholipases A2 J. Clin. Endocrinol. Metab., August 1, 1998; 83(8): 2861 - 2867. [Abstract] [Full Text]
|
|