Molecular Human Reproduction, Vol 3, 517-521, Copyright © 1997 by Oxford University Press
A Lopata, K Oliva, PG Stanton and DM Robertson
Embryos produced in an in-vitro fertilization (IVF) programme, but which
were unsuitable for transfer to patients because they originated from one
(1PN) or three pronuclear (3PN) oocytes or because they originated from two
pronuclear (2PN) oocytes but cleaved normally, were maintained in tissue
culture. The embryos that progressed to blastocytes were cultured to day 14
of development in order to study their daily output of human chorionic
gonadotrophin (HCG). Blastocysts that released large amounts of
immunoreactive HCG, which continued to increase daily during the study
period, provided the culture supernatants used in the present studies. The
heterogeneity of HCG released by blastocyst tissues on days 11 and 14 of
development was studied by a chromatofocusing method which separates the
isoforms of the gonadotrophin based on differences in their isoelectric
points. It was found that the secreted HCG was composed of several
molecular forms and that this heterogeneity changed from day 11 to 14 of
development. The early blastocyst tissues produced more acidic HCG isoforms
than the more advanced embryonic tissues. Differences in the apparent
ploidy of the blastocyst tissues studied did not affect significantly the
distribution of the HCG isoforms secreted either on day 11 or day 14 of
development. These results suggest that the bioactivity of the HCG secreted
by blastocytes may change with time and with differentiation of the
trophectoderm. In addition, the results suggest that the ploidy of early
blastocytes does not influence the nature of the HCG secreted.
JOURNAL ARTICLE
Analysis of chorionic gonadotrophin secreted by cultured human blastocysts
Department of Obstetrics and Gynaecology, University of Melbourne, Carlton, Victoria, Australia.
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