Molecular Human Reproduction, Vol 3, 651-654, Copyright © 1997 by Oxford University Press
MP Smith, SD Keay, L Hall, CR Harlow and JM Jenkins
The activity of 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD) in human
granulosa cells has been shown to be associated with the outcome of
treatment following in-vitro fertilization and embryo transfer. There are
two known isoforms of 11 beta-HSD which differ significantly in their
actions and co-factor requirements. The net activity of 11 beta-HSD which
differ significantly in their actions and co-factor requirements. The net
activity of 11 beta-HSD within the human ovary is unclear, but may be of
particular importance within the ovarian follicle in regulating possible
glucocorticoid influences on the oocyte. This study presents preliminary
information regarding establishment of techniques to identify transcripts
of the 11 beta-HSD isoforms within human granulosa cells and human cumulus
cells using reverse transcription-polymerase chain reaction. In view of the
high expression of the type 1 11 beta-HSD isoform and the possibility of
other 11 beta-HSD isoforms in the ovary, plasmid technology was used to
confirm the technique specifically identifying the known isoforms.
JOURNAL ARTICLE
The detection and confirmation of 11 beta-hydroxysteroid dehydrogenase type 1 transcripts in human luteinized granulosa cells using RT-PCR and plasmid pUC18
University of Bristol, Division of Obstetrics and Gynaecology, St. Michael's Hospital, UK.
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