Molecular Human Reproduction, Vol 3, 663-668, Copyright © 1997 by Oxford University Press
MC Johnson, M Vega, D Vantman, JL Troncoso and L Devoto
The role of angiotensin II (AngII) in ovarian steroidogenesis is not
clearly understood. In order to study its action on progesterone synthesis
and to determine which receptor subtype is involve, granulosa cells
obtained from women undergoing in-vitro fertilization were cultured for 2
or 4 days and then incubated in the presence of AngII (10(-7) M) with or
without human chorionic gonadotrophin (HCG, 10 IU/ml) for 3 or 18 h. In
cells cultured for 2 days, incubation with AngII decreased progesterone
secretion by 36%, and inhibited activity of 3 beta-hydroxysteroid
dehydrogenase (3 beta-HSD) by 87% (P < 0.05), although its expression
was not significantly reduced. However, in cells cultured for 4 days,
progesterone production was enhanced by incubation with AngII (38%), and no
change was observed in 3 beta-HSD expression. Both inhibitory and
stimulatory effects were dose- dependent. Progesterone secretion was
increased (93%) by incubation with HCG of cells cultured for 4, but not for
2 days, and no potentiation was observed with AngII. Treatment with
PD123177 completely blocked the action of AngII and decreased the
HCG-stimulated secretion of progesterone by 27%. Angiotensin type-2 (AT2)
receptor mRNA was expressed in cells cultured for 4 days. In conclusion,
AngII showed a regulatory role in in-vitro progesterone production by human
granulosa luteinized cells, modulating the activity of 3 beta-HSD. It is
likely that these actions may be mediated via membrane receptors, possibly
of the AT2 receptor family.
JOURNAL ARTICLE
Regulatory role of angiotensin II on progesterone production by cultured human granulosa cells. Expression of angiotensin II type-2 receptor
Institute of Maternal and Child Research, School of Medicine, University of Chile, San Borja-Arriaran Clinical Hospital, Santiago, Chile.
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