Molecular Human Reproduction, Vol 3, 669-676, Copyright © 1997 by Oxford University Press
D Miller
Several reports demonstrating the presence of messenger RNAs for sperm-
specific nucleoproteins, the protamines PRM-1, PRM-2, and the transition
protein TP-1 as well as beta-actin, c-MYC, HLA1, and beta 1 integrin have
challenged the accepted view of the transcriptional dormance of terminally
differentiated spermatozoa. Whatever nuclear activity the ejaculate
spermatozoon may possess, these data suggest that spermatozoa are a
repository of information regarding meiotic and post-meiotic gene
expression in the human and are likely to contain transcripts for many
homologues of genes identified from animal models, which play an essential
role in spermiogenesis. The use of ejaculate spermatozoa as a wholly
non-invasive biopsy of the spermatid should now be evaluated. At the same
time, spermatozoal RNAs require further analysis and characterization. One
explanation for their persistence in ejaculate spermatozoa is that they
serve to equilibrate imbalances in spermatozoal phenotypes brought about by
melotic recombination and segregation. Transcript exchange is likely
considering the presence of inter-cellular cytoplasmic bridges between
haploid spermatids, which also offers a route for the translocation and
expansion of non- mendelian trials linked to retrotransposon activity.
REVIEW, ACADEMIC
RNA in the ejaculate spermatozoon: a window into molecular events in spermatogenesis and a record of the unusual requirements of haploid gene expression and post-meiotic equilibration
Centre for Reproduction Growth and Development, University of Leeds, Leeds General Infirmary, UK.
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