Molecular Human Reproduction, Vol 4, 1-8, Copyright © 1998 by Oxford University Press
AJ Jakimiuk, SR Weitsman, PR Brzechffa and DA Magoffin
Polycystic ovary syndrome (PCOS) is a common reproductive disorder
characterized by arrested follicular development prior to selection of a
dominant follicle. Dominant follicles produce large amounts of oestradiol
but PCOS follicles do not. With several potential aromatase (P450AROM)
inhibitors in follicular fluid, the question arises whether P450AROM is
expressed in PCOS granulosa cells, but the activity is inhibited, or
whether P450AROM is not expressed in PCOS. The purpose of the present study
was to determine whether P450AROM mRNA expression is altered in PCOS and to
correlate P450AROM mRNA expression in individual follicles with aromatase
stimulatory bioactivity and oestradiol in the follicular microenvironments.
P450AROM mRNA was measured in individual follicles from 16 PCOS and 48
regularly cycling control women by quantitative polymerase chain reaction
(PCR) and correlated with follicular fluid oestradiol concentrations and
aromatase stimulating bioactivity measured by the rat granulosa cells
aromatase bioassay. Follicular fluid oestradiol was low in all control
follicles <7 mm in diameter. Some follicles > or = 7 mm contained
elevated oestradiol values (P < 0.01) and all had an
androstenedione:oestradiol ratio of <4. Only in granulosa cells from
follicles > or = 7 mm with an androstenedione:oestradiol ratio of <4
were P450AROM mRNA levels increased (P < 0.05). These same follicles
also contained increased levels of aromatase stimulating bioactivity
whereas follicles <7 mm or with androstenedione:oestradiol ratio of
>4 contained little or no bioactivity. All PCOS follicles contained low
levels of oestradiol, P450AROM mRNA and aromatase stimulating bioactivity
similar to size- matched control follicles. These data indicate that
P450AROM mRNA expression and oestradiol production begin in developing
follicles when they reach approximately 7 mm in diameter. Oestradiol
production is low in PCOS follicles because there is insufficient aromatase
stimulating bioactivity to increase P450AROM mRNA expression.
JOURNAL ARTICLE
Aromatase mRNA expression in individual follicles from polycystic ovaries
Department of Obstetrics and Gynecology, Second Clinic of Surgical Gynecology, University School of Medicine, Lublin, Poland.
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