Molecular Human Reproduction, Vol 4, 101-106, Copyright © 1998 by Oxford University Press
D Tsatas, MS Baker, EK Moses and GE Rice
The plasminogen activation cascade is thought to play a critical role in
labour-associated remodelling events, such as fetal membrane rupture and
placental separation. The aim of this study was to quantify, by Northern
analysis, the gene expression of urokinase plasminogen activator (UPA),
urokinase receptor (UPAR) and plasminogen activator inhibitor type-2
(PAI-2) in human gestational tissues. Amnion, choriodecidua and placenta
were collected from women before, during and after spontaneous-onset labour
at term. The expression of UPAR mRNA was significantly (P < 0.05)
increased in amnion tissue during and after labour and delivery, compared
with the before-labour group. In contrast, UPAR gene expression in
choriodecidua and placenta was not significantly altered in association
with labour onset. PAI-2 mRNA expression was also significantly (P <
0.05) increased in amnion after labour. No statistically significant
differences were observed in choriodecidua or placenta PAI-2 mRNA with
labour onset. Neither was any significant effect of labour status on UPA
mRNA identified in any of the tissues examined. This study is the first to
describe a significant increase in UPAR and PAI-2 gene expression in human
amnion tissue with labour. These data are consistent with the hypothesis
that, during labour, up-regulation of UPAR expression in amnion serves to
localize active UPA at the cell surface, thereby increasing proteolytic
activity in fetal membranes. Increased PAI-2 in amnion after labour may
provide a regulatory 'switch' to cease further proteolysis in this tissue
type. In conclusion, the data obtained support the proposal that the
plasminogen activation cascade contributes to the rupture of fetal
membranes during active labour.
JOURNAL ARTICLE
Gene expression of plasminogen activation cascade components in human term gestational tissues with labour onset
Department of Perinatal Medicine, Perinatal Research Centre, Royal Women's Hospital, Carlton, Victoria, Australia.
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