Molecular Human Reproduction, Vol 4, 17-25, Copyright © 1998 by Oxford University Press
CN Tomes, R Carballada, DF Moses, DF Katz and PM Saling
It has long been known that seminal plasma contains factors that influence
the fertilizing capacity of spermatozoa in many different ways. However,
little is understood of the biochemical cascades triggered when spermatozoa
and seminal plasma interact. In this study, we examined how incubation with
seminal plasma affected protein tyrosine phosphorylation in human
spermatozoa. Increased protein tyrosine phosphorylation is a hallmark of
sperm capacitation in several mammalian species, including human. Seminal
plasma blocks protein tyrosine phosphorylation when added to washed,
non-capacitated spermatozoa. Removal of seminal plasma and incubation in
capacitating medium led to partial recovery of the tyrosine phosphorylation
cascade. Addition of seminal plasma to a suspension of spermatozoa
previously incubated for 5 h under capacitating conditions decreased the
level of tyrosine phosphorylation on all proteins in a dose-dependent
manner. In this case, the phosphotyrosine signal did not increase upon
removal of seminal plasma followed by overnight incubation in fresh
capacitating media, indicating that removal of seminal plasma was necessary
but not sufficient for protein tyrosine phosphorylation to occur. These
results indicate that human seminal plasma contains factors that influence
the tyrosine phosphorylation status of human spermatozoa.
JOURNAL ARTICLE
Treatment of human spermatozoa with seminal plasma inhibits protein tyrosine phosphorylation
Department of Obstetrics and Gynecology, Duke University Medical Center, Durham, North Carolina 27710, USA.
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