Molecular Human Reproduction, Vol 4, 61-66, Copyright © 1998 by Oxford University Press
R Weissenberg, A Aviram, R Golan, LM Lewin, J Levron, I Madgar, J Dor, G Barkai and B Goldman
Routine semen analysis in an infertile patient revealed severe
teratospermia associated with malformation of head and tail in 100% of the
sperm cells. Flow cytometry and fluorescence in-situ hybridization (FISH)
were shown to supplement routine semen analysis by providing information on
the sperm chromatin. Using flow cytometry, propidium iodide-stained
spermatozoa from the same sperm sample were compared with a normal
reference pool, and with human lymphocytes. The results point to a
population of diploid sperm cells rather than to mature haploid
spermatozoa. Numerical chromosomal abnormalities of the spermatozoa were
subsequently evaluated using FISH. A total of 1000 sperm cells were scored
for X and Y chromosomes, and an additional 1128 sperm cells for chromosome
18. Aneuploidy of chromosomes X and Y was revealed in 96.9% of the cells
and of chromosome 18 in 90.3% of the cells. Non-disjunction of chromosome X
and Y in meiosis I and II occurred in 54.8 and 2.7% of the sperm cells
respectively. Non- disjunction in both meiosis I and II occurred in 39.4%
of the sperm cells. A normal haploid pattern for chromosomes X and Y was
observed in only 3.1%, and for chromosome 18 in 9.7%, of the cells. Using
three colour FISH for the sex chromosomes and for chromosome 18, diploidy
was demonstrated in 19.4% of 500 sperm cells and aneuploidy in virtually
all sperm cells (99.2%). The use of flow cytometry and FISH in cases where
genetic and developmental chromatin abnormalities are suspected is a
valuable adjunct to other available techniques, and can guide the
clinicians to decide which samples are unsuitable for intracytoplasmic
injection.
JOURNAL ARTICLE
Concurrent use of flow cytometry and fluorescence in-situ hybridization techniques for detecting faulty meiosis in a human sperm sample
Andrology Unit, Sheba Medical Center, Tel Hashomer, Ramat Gan, Israel.
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